岷江百合蛋白激酶基因lilyABC1的克隆及在非生物胁迫下的表达特性分析

以野生型岷江百合（Lilium regale Wilson）为材料，克隆岷江百合蛋白激酶基因lilyABC1，分析其在非生物胁迫下的表达特性，为百合抗逆育种提供候选基因。结果表明：lilyABC1基因全长cDNA序列为2 333 bp，其开放阅读框（ORF）为2 007 bp。该基因编码668个氨基酸，预测lilyABC1蛋白分子量为74.84 kD，等电点为5.46，含有一个高度保守的结构域--STYKc。lilyABC1基因在岷江百合的叶片、鳞片、花蕾组织中均有表达，表达量依次为叶 > 花蕾 > 鳞片，其中不同时期的花蕾中的表达量基本一致。lilyABC1的表达受到NaCl、低温、高温以及黑暗胁迫的诱导，对PEG-6000的模拟干旱处理不敏感，表明lilyABC1基因可能对岷江百合适应高盐、低温和黑暗等非生物胁迫具有一定作用。

Cloning of the lilyABC1 Protein Kinase Gene and Its Expression Patterns Under Abiotic Stresses in Lilium regale

LilyABC1，a gene encoding protein kinase，was cloned and its expression patterns in Lilium regale Wilson under abiotic stresses were dissected for the purpose of providing a candidate gene for lily breeding for resistance to various abiotic stresses. Plant material of wild Lilium regale Wilson was used to clone the full-length cDNA sequence of lilyABC1 using RACE technology in combination with cDNA library. A number of appropriate bioinformatics softwares were used to assay the structure of cDNA sequence and the function of deduced lilyABC1. The real-time quantitative PCR（qRT-PCR）method was applied to determine lilyABC1 expression patterns in specific tissues under different abiotic stresses including PEG-6000，NaCl，low temperature，high temperature and darkness. The results showed that thelilyABC1 cDNA was 2 333 bp with a 2 007 bp open readings frame in full-length. The sequence of the lilyABC1 consists of 668 amino acids with a molecular mass of 74.84 kD. The pI value of this protein is 5.46 and containing STYKc conserved domain. The expression levels of lilyABC1 were ranked as leaf > flower bud > bulbs；There was no obvious expression difference among different development stages of the flower buds. The expression of LilyABC1 was up-regulated by NaCl，low temperature，high temperature and darkness，but it was not sensitive to the PEG-6000. Therefore，lilyABC1 might have contributed to its resistance to abiotic stresses such as NaCl，low temperature，high temperature，dark and drought.