中国水仙NtPLATZ1正、反义植物表达载体构建及转化烟草的研究

通过构建中国水仙锌指蛋白基因NtPLATZ1正义和反义两种植物表达载体,并用冻融法将其导入农杆菌EHA105菌株,介导遗传转化烟草,对遗传转化的烟草再生植株进行PCR-Southernblot和RT-PCR检测,研究NtPLATZ1的功能。结果表明成功将NtPLATZ1转化烟草,获得5株阳性转基因植株。NtPLATZ1在转基因烟草中过表达能抑制烟草的生长,抑制表达可促进烟草生长。

Research on Construction of Sense and Antisense Plant Expression Vectors of NtPLATZ1 Gene in Narcissus tazetta var. chinensis and Genetic Transformation to Tobacco Plants

To characterize the function of NtPLATZ1 gene from Narcissus tazetta var. chinensis，sense and antisense plant expression vectors were generated and transferred into Agrobacterium tumefaciens strain EHA105. Then vectors were transformed into tobacco plants by using Agrobacterium-mediated method. Regenerated plants of tobacco were detected by PCR-Southern blot and RT-PCR. The results showed that the sense and antisense genes were successfully integrated into the genomes of tobacco cells and expressed in transgenic tobacco plantlets. Transgenic plants were obtained successfully，which were inhibited the growth when the gene was over expression，and were promoted the growth when the gene was inhibited expression.