| 兔抗O型口蹄疫VLPs酶标抗体的制备及初步应用 |
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| 引用本文： | 贺佳慧,白满元,郭慧琛,孙世琪,张小丽,马小军. 兔抗O型口蹄疫VLPs酶标抗体的制备及初步应用[J]. 动物医学进展, 2019, 0(1): 20-24 |
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| 作者姓名： | 贺佳慧 白满元 郭慧琛 孙世琪 张小丽 马小军 |
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| 作者单位： | 甘肃农业大学动物医学院;中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室 |
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| 基金项目： | 国家重点研发计划项目(2017YFD0500906;2016YFD0500700;2017YFD0501100);中央级科研院所基本科研业务费专项(1610312016002;1610312017010) |
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| 摘 要： | 为制备兔抗O型口蹄疫病毒样颗粒(VLPs)的酶标抗体,进一步建立O型口蹄疫病毒的ELISA检测方法,通过原核表达、体外纯化和组装O型口蹄疫VLPs,免疫家兔制备兔抗O型口蹄疫VLPs高免血清,并采用亲和层析法纯化IgG,改良过碘酸钠法制备兔抗O型口蹄疫VLPs的酶标抗体(rabbit anti-type O foot-and-mouth disease IgG-HRP)。结果显示,兔抗O型口蹄疫VLPs高免血清效价达1∶4 096,纯化后的IgG纯度达90%,效价达1∶512 000。表明获得了高纯度的兔抗O型口蹄疫VLP的酶标抗体,可用于O型口蹄疫的ELISA检测。
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| 关 键 词： | 口蹄疫病毒 病毒样颗粒 IgG纯化 酶标抗体 |
Preparation of Rabbit Anti-type O Foot-and-mouth Disease Virus VLPs IgG-HRP |
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| HE Jia-hui,BAI Man-yuan,GUO Hui-chen,SUN Shi-qi,ZHANG Xiao-li,MA Xiao-jun. Preparation of Rabbit Anti-type O Foot-and-mouth Disease Virus VLPs IgG-HRP[J]. Progress In Veterinary Medicine, 2019, 0(1): 20-24 |
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| Authors: | HE Jia-hui BAI Man-yuan GUO Hui-chen SUN Shi-qi ZHANG Xiao-li MA Xiao-jun |
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| Affiliation: | (College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu,730070,China;State Key Laboratory of VeterinaryEtiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Lanzhou,Gansu,730046,China) |
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| Abstract: | HE Jia-hui;BAI Man-yuan;GUO Hui-chen;SUN Shi-qi;ZHANG Xiao-li;MA Xiao-jun(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu,730070,China;State Key Laboratory of VeterinaryEtiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Lanzhou,Gansu,730046,China) |
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| Keywords: | FMDV VLPs IgG purification IgG-HRP |
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