| 金叶弯刺蔷薇×山刺玫遗传连锁图谱的初步构建 |
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| 引用本文： | 杨树华,李世超,贾瑞冬,赵 鑫,金茂勇,李凤杰,葛 红.金叶弯刺蔷薇×山刺玫遗传连锁图谱的初步构建[J].园艺学报,2015,42(12):2526-2534. |
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| 作者姓名： | 杨树华 李世超 贾瑞冬 赵 鑫 金茂勇 李凤杰 葛 红 |
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| 作者单位： | 1中国农业科学院蔬菜花卉研究所，北京 100081；2北京市大兴区园林绿化局，北京 102600 |
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| 基金项目： | 国家‘863’计划项目（2011AA100208）；农业部‘948’项目（2011-G17）；国家科技支撑计划项目（2012BAD01B0704）北京市大兴区与中国农业科学院科技合作项目；农业部园艺作物生物学与种质创制重点实验室项目 |
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| 摘 要： | 以金叶弯刺蔷薇(Rosa beggeriana‘Aurea’,叶色突变材料)和山刺玫(R.davurica)为杂交亲本建立F1群体,利用AFLP和SSR分子标记进行遗传连锁图谱构建。结果表明:124个AFLP标记、25个SSR标记及1个形态标记定位于父、母本遗传连锁图谱的13个连锁群上,其中92个分子标记定位于金叶弯刺蔷薇7个连锁群,遗传距离覆盖度为607.2 c M,金叶突变性状被定位于第4连锁群;57个分子标记定位于山刺玫6个连锁群,遗传距离覆盖度为437.1 c M。两类分子标记的总作图效率达到69.6%。发生偏分离的分子标记数量为44个,偏分离率为29.5%。
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| 关 键 词： | 金叶弯刺蔷薇 山刺玫 遗传作图 AFLP标记 SSR标记 |
Primary Construction of a Genetic Linkage Map of Rosa beggeriana‘Aurea’× R. Davurica |
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| YANG Shu-hua,LI Shi-chao,JIA Rui-dong,ZHAO Xin,JIN Mao-yong,LI Feng-jie,GE Hong,.Primary Construction of a Genetic Linkage Map of Rosa beggeriana‘Aurea’× R. Davurica[J].Acta Horticulturae Sinica,2015,42(12):2526-2534. |
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| Authors: | YANG Shu-hua LI Shi-chao JIA Rui-dong ZHAO Xin JIN Mao-yong LI Feng-jie GE Hong |
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| Institution: | 1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Forestry and Landscape Bureau of Daxing District of Beijing Municipality，Beijing 102600，China |
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| Abstract: | The F1 population of Rosa beggeriana‘Aurea’× R. davurica was established for the construction of genetic linkage map using AFLP and SSR markers. The results showed that 124 AFLP markers，25 SSR markers and 1 morphological marker were located in total 13 linkage groups of the parents. There were 92 markers covered the genetic distances of 607.2 cM in 7 linkage groups of R. beggeriana‘Aurea’，which the yellow-leaf mutant trait was located in the 4th linkage group. In addition，there were 57 markers covered the genetic distances of 437.1 cM in 6 linkage groups of R. davurica. The total mapping efficiency reached 69.6% and 44 distorted markers were detected with the distortion rate of 29.5%. |
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| Keywords: | Rosa beggeriana Rosa davurica genetic mapping AFLP SSR |
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