高效液相色谱-质谱法分析金花葵花中金丝桃苷

目的]建立高效液相色谱-质谱法,分析金花葵花中金丝桃苷。方法]以乙腈-浓度0.1%甲酸为流动相,流速0.6 ml/min,经反相色谱柱Lichrospher C18(250 mm×4.6 mm,5μm)分离后进行质谱分析,采用正负电喷雾离子源同时进行信号采集。结果]经HPLC/MS定性鉴定,金花葵花中含有金丝桃苷,含量测定的线性范围为10.0~300.0μg/ml,平均回收率为101.27%。结论]该方法简便、快速、灵敏度高,可用于分析金花葵花中金丝桃苷的分离测定。

Analysis of Hyperoside in Flower of Hibiscus manihot L.by HPLC-Mass Spectrometry

Objective]The hyperoside in flower of Hibiscus manihot L.was measured by HPLC-Mass spectrometry.Method] By means of acetonitrile-0.1% formic acid as mobile phase with flowing rate at 0.6 ml/min,exploiting Lichrospher C18(250mm×4.6mm,5μm),the tandem mass spectral acquisition was done in positive and negative electropray ionization.Result] The flower studied contained hyperoside.The linear range of this method was from 10.0 to 300.0 μg/ml.The average recovery was 101.27%.Conclusion] The method could be applied to determine hyperoside in the flower of Hibiscus manihot L.because of its briefness,rapidity,good sensitivity and recovery.