灭活鰤鱼诺卡氏菌对乌醴非特异性免疫指标的影响

应用浓度为108CFU/ml的0.4%甲醛灭活鰤鱼诺卡氏菌Nocardia seriolea，经超声波破碎后对乌鳢进行腹腔注射，剂量为0.5 ml/尾，对照组同法等量注射灭菌生理盐水。分别于实验第0、3、6、9、12、15、18、21天抽取乌鳢血液，测定其血细胞数、血清总蛋白含量及血清碱性磷酸酶、酸性磷酸酶、超氧化物歧化酶、溶菌酶活性。结果显示，注射灭活鱼师鱼诺卡氏菌后，乌鳢血细胞数呈先升高后降低的变化趋势，在实验第12天时注射组血细胞数显著高于对照组；血清总蛋白含量在实验第9天后均高于对照组；血清碱性磷酸酶、酸性磷酸酶、超氧化物歧化酶和溶菌酶等活性在实验时间内均发生了显著的变化。研究结果表明，灭活菌苗的刺激能引起乌醴血细胞的增殖以及血清中各非特异性免疫指标的变化，从而提高机体自身的免疫保护力。

Effects of inactivated Nocardia seriolae on the nonspecific immune indexes of Channa argus

Experiments were carried out to evaluate the influences of inactivated Nocardia seriolae on the nonspecific immune indexes of Channa argus. One hundred healthy fish (C. argus) at body weight of 300~400 g were divided randomly into immunized and control groups. Each individual in the immunized group was intraperitoneally injected with 0.5 ml 1.0×108CFU/ml N.seriolae which was inactivated by formalin and ultrasonic disruption, while each individual was intraperitoneally injected with 0.5 ml sterile saline solution in the control group. After the injection, blood samples were taken from the fish caudal vein on days 0, 3, 6, 9, 12, 15, 18, and 21 to test the changes of nonspecific immune indexes such as haematocyte number, serum total protein, alkaline phosphatase(AKP), acid phosphatase(ACP), superoxide dismutase(SOD) and lysozyme activities. The results showed that haematocyte number in immunized C. argus increased at first and was significantly higher than that in control group on day 12, but then decreased. Content of serum total protein of the immunized C. argus was significantly higher than that in the control group on day 9. Alkaline phosphatase (AKP), acid phosphatase (ACP), superoxide dismutase (SOD) and lysozyme activities showed significant changes during the experiment. All these findings indicate that inactivated N. seriolae may enhance the non-specific immunity of C. argus, producing a significant protection against the pathogen bacteria.