| 一株新型鸭源呼肠孤病毒(TH11株)的分离与鉴定 |
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| 引用本文： | 陈宗艳,朱英奇,王世传,李传峰,李露,王玢缤,付玉志,高景鹏,王超,刘光清.一株新型鸭源呼肠孤病毒(TH11株)的分离与鉴定[J].中国动物传染病学报,2012(1):10-15. |
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| 作者姓名： | 陈宗艳 朱英奇 王世传 李传峰 李露 王玢缤 付玉志 高景鹏 王超 刘光清 |
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| 作者单位： | 中国农业科学院上海兽医研究所;安徽省全椒县畜牧兽医局 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(2012JB06,2012JB13);公益性农业科研专项(201003012);“863”计划(2011AA10A200) |
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| 摘 要： | 本文从太湖流域某养鸭场分离到一株呼肠孤病毒(Duck reovirus,DRV)TH11株,该病毒感染的病鸭主要表现软脚和拉稀等主要症状,剖检病变以肝脏出血和脾脏坏死为主要特征。电镜鉴定结果显示,该病原具有呼肠孤病毒的典型形态学特征。动物回归试验和RT-PCR扩增结果证明,该株病毒无论是在致病性方面,还是在基因序列方面都不同于以往的番鸭呼肠孤病毒,而是一株对多品种鸭具有较高致病性和病死率的新型鸭呼肠孤病毒。
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| 关 键 词： | 鸭源呼肠孤病毒 动物回归试验 电镜 RT-PCR |
ISOLATION AND CHARACTERIZATION OF A NEW TYPE OF DUCK REOVIRUS |
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| CHEN Zong-yan,ZHU Ying-qi,WANG Shi-chuan,LI Chuan-feng,LI Lu,WANG Fen-bin,FU Yu-zhi,GAO Jing-peng,WANG Chao,LIU Guang-qing.ISOLATION AND CHARACTERIZATION OF A NEW TYPE OF DUCK REOVIRUS[J].Chinese Journal of Animal Infectious Diseases,2012(1):10-15. |
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| Authors: | CHEN Zong-yan ZHU Ying-qi WANG Shi-chuan LI Chuan-feng LI Lu WANG Fen-bin FU Yu-zhi GAO Jing-peng WANG Chao LIU Guang-qing |
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| Institution: | 1(1.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;2.Quanjiao Animal Husbandry and Veterinary bureau,Anhui Province,Quanjiao 239500,China) |
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| Abstract: | A new infectious disease emerged on a ducks farm in Jiangsu Province.The sick ducks showed weakness in legs and diarrhea and pathological findings included necrotic foci in the spleens and hemorrhage in the livers.A virus was isolated from the liver and spleen of a dead duck.The virus isolate designated Duck reovirus(DRV) TH11 was demonstrated different from those previously documented in pathogenecity in ducks,genetic analysis in RT-PCR and morphology under electron microscopy.Therefore,the virus isolate DRV-TH11 represented a new type of duck reovirus. |
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| Keywords: | Duck reovirus animal regression experiment electron microscopy RT-PCR |
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