| Establishment of Plant Acceptor System for Hyperosmosis Transformation. |
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| 引用本文： | HuXinx YiZili ZhouHualan JinLongxin QuanHua. Establishment of Plant Acceptor System for Hyperosmosis Transformation.[J]. 农业科学与技术, 2001, 2(1): 17-21 |
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| 作者姓名： | HuXinx YiZili ZhouHualan JinLongxin QuanHua |
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| 作者单位： | [1]HunanAgriculturalUniversity,Changsha,Hunan410128 [2]HunanAcademyofAgriculturalSciences,Changsha,Hunan410125 [3]WsetHunanInstituteofAgricultualSciences,Jishou,Hunan416000 |
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| 摘 要： | Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells fo calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypoosmoticum respectively.
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| 关 键 词： | 转基因植物 外植体 受体组织 培养基 高渗处理 愈伤组织 |
Establishment of Plant Acceptor System for Hyperosmosis Transformation. |
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| Hu Xinxi,Yi Zili,Zhou Hualan,Jin Longxin,Quan Hua. Establishment of Plant Acceptor System for Hyperosmosis Transformation.[J]. Agricultural Science ＆ Technology, 2001, 2(1): 17-21 |
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| Authors: | Hu Xinxi Yi Zili Zhou Hualan Jin Longxin Quan Hua |
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| Abstract: | Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker, and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor. The cells of calli were still vigorous when treated wity 0.75 mol/L hyperosmotic solution for 4 hours. The differentiation rates of calli varied from 7.5% to 93.3% in different media. The bud differentiation was apparently inhibited by hyperosmotic treatments. O.75mol/L sucrose hypertonic solution and O.2mol/L CaCl2 solution were favorable hyperosmoticum and hypoosmoticum respectively. |
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