利用SRAP标记划分甘蓝型油菜杂种优势群

利用SRAP分子标记研究了来自国内外50个甘蓝型油菜(Brassica napusL.)品种的遗传变异,从120对SRAP引物组合中,筛选出扩增带型稳定的15对引物进行遗传多样性分析,共获得了140个多态性标记,平均每个位点检测到的等位基因变异数为9.3个,变化范围2~16个;平均PIC值为0.529,变化范围为0.27~0.73;遗传距离范围为0.0838~0.4326。经遗传多样性分析和聚类分析,将50份供试材料分为6个类群,群间遗传距离大于群内遗传距离,基本反映了品种的地源差异,表明SRAP标记可以有效地区分甘蓝型油菜的遗传多样性,划分结果客观合理。

Classification of Heterosis Groups for Rapeseed(Brassica napus)by SRAP Markers

In the present study,15 of 120 SRAP primer combinations were screened out to analyze the genetic diversity among 50 rapeseed(Brassica napus L.) varieties,the genetic diversity between two varieties was defined with genetic distance(GD) by Nei M method,and all cultivars were analyzed by clustering method.The results showed that 140 SRAP markers were obtained.The number of alleles per SRAP locus averaged 9.3 with the range from 2 to 16.The value of polymorphism information content(PIC) for all SRAP primers varied from 0.27 to 0.73 with an average of 0.529.The GD among the 50 varieties ranged between 0.0838~0.4326.The UPGMA clustering analysis divided 50 varieties into 6 groups.After classification,the average GD among groups was higher than that within groups,which was approximately in accordance with their diversity of cultural area.So the classification of the heterosis groups for rapeseed(Brassica napus) by SRAP makers in this work is appropriate.