Rapid detection of feline morbillivirus by a reverse transcription
loop-mediated isothermal amplification |
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| Authors: | Rie KOIDE Shoichi SAKAGUCHI Makoto OGAWA Takayuki MIYAZAWA |
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| Affiliation: | 1)Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606–8507, Japan;2)Ogawa Pet Clinic, 6–12 Shino-cho, Kameoka, Kyoto 621–0822, Japan |
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| Abstract: | Feline morbillivirus (FmoPV) is an emerging virus in domestic cats andconsidered to be one of the causes of chronic renal failure in cats. In this study, weestablished a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assayfor the detection of FmoPV. The results indicated that the detection limit of the assaywas 10 50% tissue culture infective dose (TCID50)/ml in theoriginal sample, and sensitivity of the assay was calculated as 0.12 TCID50 perone RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infectedwith FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detectionof FmoPV, and thus, it would be a reliable detection method for FmoPV. |
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| Keywords: | detection feline feline morbillivirus reverse transcription loop-mediated isothermal amplification |
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