Localization of connexin 32 in spontaneous liver lesions of
mice |
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| Authors: | Isao IGARASHI Toshihiko MAKINO Kiyonori KAI Munehiro TERANISHI Wataru TAKASAKI Hiroshi SATOH Kazuhisa FURUHAMA |
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| Institution: | 1)Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd., Kitakasai, Edogawa, Tokyo 134–8630, Japan;2)Cooperative Department of Veterinary Medicine, Iwate University, Morioka, Iwate 020–8550, Japan;3)United Graduate School of Veterinary Science, Gifu University, Gifu 501–1193, Japan |
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| Abstract: | We examined the localization of connexin 32 (Cx32), a component of gap
junctions, in 24-month-old male B6C3F1 mice with spontaneously occurring hepatocellular
altered foci or tumors. Immunohistochemically, Cx32-staining intensity in cell-to-cell
membranes of altered hepatocytes was decreased in eosinophilic foci and increased in
basophilic foci as compared to those in intact hepatocytes. These alterations were
enhanced in adenomas and carcinomas with both eosinophilic and basophilic cytoplasm. In
cell membranes facing on the sinusoidal portions, the intensities increased in all
lesions. Image analyses confirmed that the spot areas of Cx32 were decreased in
eosinophilic foci, but increased in basophilic foci, adenomas and carcinomas. These
results demonstrate that Cx32 shows different expression in different types of hepatic
lesions. |
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| Keywords: | connexin immunohistochemistry liver lesion mouse |
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