| 赤点石斑鱼神经坏死病毒MCP基因原核表达条件优化(英文) |
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| 引用本文： | 苏友禄,冯娟,孙秀秀,郭志勋,闫云锋,黄剑南. 赤点石斑鱼神经坏死病毒MCP基因原核表达条件优化(英文)[J]. 农业科学与技术, 2008, 9(6): 59-63 |
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| 作者姓名： | 苏友禄 冯娟 孙秀秀 郭志勋 闫云锋 黄剑南 |
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| 作者单位： | 苏友禄,冯娟,郭志勋,黄剑南,SU You-lu,FENG Juan,GUO Zhi-xun,HUANG Jian-nan(中国水产科学研究院南海水产研究所,广东广州,510300);孙秀秀,SUN Xiu-xiu(华南农业大学兽医学院,广东广州,510642);闫云锋,YAN Yun-feng(中国水产科学研究院南海水产研究所,广东广州,510300;上海海洋大学生命科学与技术学院,上海,200090) |
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| 摘 要： | [Objective] To optimize the prokaryotic expression of MCP gene of red-spotted grouper nervous necrosis virus. [Method] The MCP gene was amplified from red-spotted grouper nervous necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21(DE3)plysS to express proteins with induction in different media, at different pH, or at different temperatures. [Result] The expression level of recombinant bacteria reached a peak with induction under the following condition: SOB or LB medium, pH 7.0, 37 ℃ while the fusion protein was about 44.5 kD in molecular weight. [Conclusion] This study provided a basis for the development of RGNNV-MCP vaccine.
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| 关 键 词： | Epinephelus akaara NNV MCP Expression conditions |
Optimization for Prokaryotic Expression of MCP Gene of Red-spotted Grouper Nervous Necrosis Virus |
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| SU You-lu,FENG Juan,SUN Xiu-xiu,GUO Zhi-xun,YAN Yun-feng,HUANG Jian-nan. Optimization for Prokaryotic Expression of MCP Gene of Red-spotted Grouper Nervous Necrosis Virus[J]. Agricultural Science ＆ Technology, 2008, 9(6): 59-63 |
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| Authors: | SU You-lu FENG Juan SUN Xiu-xiu GUO Zhi-xun YAN Yun-feng HUANG Jian-nan |
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| Affiliation: | 1. South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300; 2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642; 3. College of Life Science and Technology, Shanghai Ocean University, Shanghai 200090 |
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| Abstract: | [Objective] To optimize the prokaryotic expression of MCP gene of red-spotted grouper nervous necrosis virus. [Method] The MCP gene was amplified from red-spotted grouper nervous necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21(DE3)plysS to express proteins with induction in different media, at different pH, or at different temperatures. [Result] The expression level of recombinant bacteria reached a peak with induction under the following condition: SOB or LB medium, pH 7.0, 37 ℃ while the fusion protein was about 44.5 kD in molecular weight. [Conclusion] This study provided a basis for the development of RGNNV-MCP vaccine. |
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| Keywords: | Epinephelus akaara NNV MCP Expression conditions |
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