| 草菇冷诱导Cor3基因实时荧光定量PCR标准品质粒和标准曲线的构建 |
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| 引用本文： | 汪虹,陈明杰. 草菇冷诱导Cor3基因实时荧光定量PCR标准品质粒和标准曲线的构建[J]. 食用菌学报, 2007, 14(3): 16-19 |
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| 作者姓名： | 汪虹 陈明杰 |
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| 作者单位： | 上海市农业遗传育种重点实验室,上海市农业科学院食用菌研究所,上海,201106 |
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| 基金项目： | 上海农科院青年科学基金;上海市科学技术委员会专项基金重点项目 |
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| 摘 要： | A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.
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| 关 键 词： | 草菇 冷诱导 Cor3基因 定量PCR 质粒标准品 标准曲线 |
| 文章编号： | 1005-9873（2007）03-0016-04 |
| 收稿时间： | 2007-05-18 |
| 修稿时间： | 2007-07-15 |
Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Cor3 Gene from Volvariella volvacea |
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| WANG Hong,CHEN Mingjie. Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Cor3 Gene from Volvariella volvacea[J]. Acta Edulis Fungi, 2007, 14(3): 16-19 |
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| Authors: | WANG Hong CHEN Mingjie |
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| Abstract: | A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea. |
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| Keywords: | Volvariella volvacea Cold induction Cor3 gene Quantitative PCR Plasmid Standard curve |
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