| 牛去核卵母细胞能够被孤雌激活并发育到桑椹胚 |
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| 引用本文： | 王志伟,胡勇策,马晓玲,刘文强,殷吉庆,云彦,雷安民.牛去核卵母细胞能够被孤雌激活并发育到桑椹胚[J].农业生物技术学报,2010,18(1):87-91. |
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| 作者姓名： | 王志伟 胡勇策 马晓玲 刘文强 殷吉庆 云彦 雷安民 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西省干细胞工程技术研究中心,陕西省农业分子生物学重点实验室,杨凌,712100 |
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| 基金项目： | 西北农林科技大学留学回国人员科研启动项目 |
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| 摘 要： | 为了探讨实验牛去核卵母细胞能否被激活,及其激活后的发育能力,本研究利用化学激活法对牛去核卵母细胞进行孤雌激活,然后在体外颗粒细胞饲养层上进行培养,同时在不同的时间采用免疫荧光技术对α-微管蛋白(α-tubulin)进行染色,以观察去核卵母细胞与正常卵母细胞中微管分布动态变化的差别.结果表明去核卵母细胞经激活后在体外培养能够发育到桑椹胚,去核卵母细胞内α-tubulin的分布在分裂初期没有分区现象,对照组未去核卵母细胞内的α-tubulin抗体出现了分区现象.
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| 关 键 词： | 牛卵母细胞 Hoechst染色 激光共聚焦 |
Derivation Morula from Bovine Enucleated Oocytes Activated by Parthenogenesis |
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| Wang Zhiwei Hu Yongce Ma Xiaoling Liu Wenqiang Yin Jiqing Yun yan Lei Anmin Northwest Agriculture & Forestry University,College of Veterinary Medicine,Shaanxi Stem Cell Engineering , Technology Research Center,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling.Derivation Morula from Bovine Enucleated Oocytes Activated by Parthenogenesis[J].Journal of Agricultural Biotechnology,2010,18(1):87-91. |
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| Authors: | Wang Zhiwei Hu Yongce Ma Xiaoling Liu Wenqiang Yin Jiqing Yun yan Lei Anmin Northwest Agriculture & Forestry University College of Veterinary Medicine Shaanxi Stem Cell Engineering Technology Research Center Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling |
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| Institution: | Wang Zhiwei Hu Yongce Ma Xiaoling Liu Wenqiang Yin Jiqing Yun yan Lei Anmin * Northwest Agriculture & Forestry University,College of Veterinary Medicine,Shaanxi Stem Cell Engineering , Technology Research Center,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling,712100 |
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| Abstract: | |
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| Keywords: | Bovine oocytes Hoechst staining Laser scanning confocal |
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