Comparison of latex agglutination,enzyme-linked immunosorbent assay,and indicator plants for detection of potato viruses S and X in potatoes |
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| Authors: | Gary D. Franc Ernest E. Banttari |
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| Affiliation: | 1. Department of Plant Pathology and Weed Science, Colorado State University, 80S23, Fort Collins, CO
2. Department of Plant Pathology, University of Minnesota, 55108, St. Paul, MN
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| Abstract: | Potato virus S (PVS) was consistently detected by the enzyme-linked immunosorbent assay (ELISA) and the latex agglutination test (LAT) at dilutions (PVS sap: healthy sap) of 1:499 and 1:19, respectively. Mechanical inoculation ofNicotiana debneyi gave variable results with detection possible 38% of the time at dilutions of 1:499. Potato virus X (PVX) was consistently detected by ELISA and LAT at dilutions (PVX sap: healthy sap) of 1:199 and 1:49, respectively. Mechanical inoculation ofGomphrena globosa with PVX resulted in consistent detection of the virus at a dilution of 1:499. |
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