| 重组高繁殖力疫苗株H5N2(H5/PR8)病毒的制备和鉴定 |
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| 引用本文： | 施建忠,田国彬,李雁冰,陈化兰. 重组高繁殖力疫苗株H5N2(H5/PR8)病毒的制备和鉴定[J]. 中国预防兽医学报, 2005, 27(6): 530-534 |
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| 作者姓名： | 施建忠 田国彬 李雁冰 陈化兰 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室,国家禽流感参考实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 作为禽流感疫苗株,不但要求其具有良好的免疫原性,而且要求在生产中具有良好的生长特性,现用的A/Turkey/England/N28/73(H5N2)疫苗株,血凝价28,生长滴度较差.本研究利用自然重组法,预制备一株高繁殖力的预备疫苗株,使它的表面基因血凝素(HA)和神经氨酸酶(NA)来源于A/Turkey/England/N28/73(H5N2)毒株,并使其保持原有的良好的免疫原性,内部基因来源于流感病毒PR8,A/Puerto Rico/8/34(H1N1)毒株,并使其重组病毒赋有了流感病毒PR8高繁殖力的特性.实验结果表明,已获得一株高繁殖力的重组流感病毒H5/PR8,血凝价达到211,生长繁殖特性明显增强.抗原性分析显示,H5/PR8重组病毒与亲本毒株H5N2抗原性无明显差异;致病性分析发现,H5/PR8重组病毒的致病能力较亲本毒株H5N2有明显下降.这为疫苗株的改良奠定了坚实的基础,对禽流感灭活疫苗的生产具有重大意义.
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| 关 键 词： | 流感病毒 重组 H5N2亚型 |
| 文章编号： | 1008-0589(2005)06-0530-05 |
| 收稿时间： | 2004-07-12 |
| 修稿时间： | 2004-07-12 |
Generation and identification of a high-growth reassortant H5/PR8 influenza A virus |
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| SHI Jian-zhong,TIAN Guo-bin,LI Yan-bin,CHEN Hua-lan. Generation and identification of a high-growth reassortant H5/PR8 influenza A virus[J]. Chinese Journal of Preventive Veterinary Medicine, 2005, 27(6): 530-534 |
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| Authors: | SHI Jian-zhong TIAN Guo-bin LI Yan-bin CHEN Hua-lan |
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| Affiliation: | National Avian Influenza Reference Laboratory, Animal Influenza Lahora~ry of Ministry of Agriculture, Harbin veterinary Research Institute of CA/kS, Harbin 150001, China |
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| Abstract: | We generated a high-growth reassortant virus(H5/PR8) that contains the hemagglutinin(HA) and neuraminidase(NA) genes from the H5N2 avian influenza virus A/Turkey/England/N28/73(H5N2) and six internal genes from PR8,A/Puerto Rico/8/34(H1N1) by genetic reassortant,for evaluation as a potential vaccine candidate in poultry.The results of antigenic analysis showed that H5/PR8 reassortant was in the same antigenic group with H5N2.In addition,Pathogenicity studies showed that the H5/PR8 reassortant was not highly pathogenic for chicken.These results indicate that the high growth H5/PR8 reassortant has properties that are desirable in a vaccine seed virus and is suitable for use in the event of a H5 pandemic. |
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| Keywords: | PR8 |
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