波兰、英国油菜种子样本的健康测试及黑胫病病原菌感染分析

对来自波兰及英国的2个相同品种的油菜种子样品进行了健康测试，并重点观察了油菜黑胫病病原菌(Letosphoeria maculans/Phoma lingam)感染的程度及菌系类型。结果表明来自2个国家的种子都一定程度受微生物感染，品种间差异不大，所检测到的较多的菌属为Alternaria,Fusarium及Penicillium。黑胫病病原菌感染的百分率波兰种子样品平均为0．5％，英国种子样品平均为0.6％，菌系鉴定表明，7．1％的波兰株系及12．3％的英国株系为强致病的Tox 类型。此外，还发现健康测试前种子表面消毒可提高黑胫病病原菌的检出率。

Health Testing and Evaluation of Leptosphaeria maculans (Phoma lingam) Contamination in Brassica napus Seed Samples from Poland and England

Mycological analysis and evaluation of Leptosphaeria maculans(Phoma lingam) contamination in Brassica napus seed samples from England and Poland were conducted. The results showed that 26%～40% of the seeds were contaminated by fungi and bacteria with Alternaria,Fusarium and Penicillium as the most commonly recovered genera. P. lingam contamination in Polish and English seed samples were 0.5% and 0.6% respectively, of which 7.1% of Polish isolates and 12.3% of English ones were classified as Tox+ pathotype. Seed surface sterilization prior to seed health testing resulted in an increase of the detection of P.lingam.