| 叶酸人工抗原合成及免疫抗血清的鉴定 |
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| 引用本文： | 张会.叶酸人工抗原合成及免疫抗血清的鉴定[J].中国农学通报,2011,27(11):78-81. |
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| 作者姓名： | 张会 |
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| 作者单位： | 中国农业大学;北京京检实力可技术检测有限公司;北京市通州区新华医院保健科;内蒙古包头市林木种苗站; |
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| 摘 要： | 叶酸是人体必需的营养物质，叶酸的缺乏可以引起神经管畸形等多种疾病，建立叶酸的快速检测方法对营养强化食品的质控以及临床研究具有重要意义。本实验通过活化酯法合成叶酸-牛血清白蛋白（FA-BSA）人工免疫原，通过混合酸酐法制备叶酸-卵清蛋白（OVA）包被原。通过紫外扫描和免疫动物进行人工抗原的合成鉴定。根据BSA、OVA的含量以及人工抗原、BSA、OVA及叶酸（FA）的紫外吸收曲线在280nm处的摩尔吸收值，计算出叶酸与BSA的偶联比为1：3.84， 叶酸与OVA的偶联比为4.70。利用合成的叶酸人工抗原免疫小鼠，三免后眼眶内眦静脉采血，采用间接ELISA方法测定免疫小鼠多抗血清效价在128,000以上，叶酸对抗血清的IC50为14.82ng/ml；由此表明叶酸人工免疫原合成成功，为进一步研究叶酸单克隆抗体奠定基础。
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| 关 键 词： | 磷石膏 磷石膏 农业 土壤 生物降解 安全 |
| 收稿时间： | 9/8/2010 12:00:00 AM |
| 修稿时间： | 2010/11/17 0:00:00 |
Preparation of Folic acid artificial antigen and identification of antiserum |
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| Zhang Hui ,Wei Wen ,Song Guiping ,He Jiguo.Preparation of Folic acid artificial antigen and identification of antiserum[J].Chinese Agricultural Science Bulletin,2011,27(11):78-81. |
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| Authors: | Zhang Hui Wei Wen Song Guiping He Jiguo |
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| Institution: | Zhang Hui 1,2,Wei Wen 3,Song Guiping 4,He Jiguo 1(1 China Agriculture Uiniversity,Beijing 100083,2 Beijing Jingjian Silliker Testing Service Inc,Beijing 100193,3 Xinhua Hospital Tongzhou District,Beijing 101100,4 Institute of Seedlings,Baotou Inner Mongolia 014010) |
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| Abstract: | The purpose of this study waw to synthesize and identify folic acid(FA) artificial antigen and obtain its polyclonal antiserum.The Folic acid immunogen(FA-BSA) and coating antigen(FA-OVA) were synthesized by active ester method and mixed anhydride method.The structure of FA-BSA and FA-OVA was identified by ultraviolet scanning and the the biological identification of FA-OVA was done by mice immunization.At the same time,polyclonal antiserum was obtained and identified by ELISA.The results showed that FA was... |
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| Keywords: | folic acid antigen synthesization polyclonal antiserum identification |
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