柑橘品种对不同基因型柑橘衰退病毒的抑制及变异的影响

为研究不同柑橘品种对柑橘衰退病毒（Citrus tristeza virus，CTV）不同基因型的抑制效果，将T36、T30、VT和T3等基因型CTV的毒源分别接种于‘赛蒙斯’甜橙、‘邓肯’葡萄柚、‘墨西哥莱檬’和‘强徳勒柚’，运用RT-qPCR技术检测被接种植株中CTV复制水平的差异。结果表明，‘墨西哥莱檬’最适于CTV各基因型的复制。‘邓肯’葡萄柚中VT、T3和T36基因型的复制水平最低。4种柑橘品种对T36基因型CTV都有明显的抑制作用。通过对CTV的CP基因进行分析发现，VT基因型CTV在‘赛蒙斯’甜橙中发生了较大变异。

Suppression and Sequence Variation of Citrus tristeza virus Genotypes by Citrus Cultivars

For the preliminary study on the suppression of Citrus tristeza virus（CTV）genotypes by different citrus cultivars. Four genotypes of CTV were graft-inoculated to‘Symons’Sweet orange，‘Mexican Lime’，‘Duncan’grapefruit and‘Chandler Pummelo’. The results of RT-qPCR showed that ‘Mexican Lime’was most suitable for the survival of these four CTV genotypes. The cope levels of VT，T3 and T36 genotypes in‘Duncan’grape fruit were much more lower than in other citrus cultivars. Furthermore，T36 genotype was reduced significantly by all of the citrus cultivars. Sequence analysis indicated that CP gene of VT genotype had frequent mutation in sweet orange.