| 用SCGE技术检验铬污染对鲫鱼肾细胞DNA损伤的研究 |
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| 引用本文： | 赵守城,张娟.用SCGE技术检验铬污染对鲫鱼肾细胞DNA损伤的研究[J].河北渔业,1999(4):11-13. |
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| 作者姓名： | 赵守城 张娟 |
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| 作者单位： | 华北煤炭医学院细胞生物学教研室
(赵守城)，华北煤炭医学院细胞生物学教研室(张娟) |
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| 摘 要： | 本文应用单细胞凝胶电泳(或彗星试验)研究了铬(Cr~(+3))对鲫鱼肾细胞DNA的损伤作用。结果表明,A组(0.8mg/l)、B组(1.6mg/l)和C组3.2mg/l)均可引起DNA的损伤。带有彗尾细胞的百分比值和彗尾长度均可依铬浓度增加而上升,呈现出剂量效应关系。本实验提示,铬浓度在不超过渔业水质标准的情况下,可引起鲫鱼肾细胞DNA的损伤。
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| 关 键 词： | 氯化铬 DNA损伤 单细胞凝胶电泳 |
Study on the Effects of Chromium (Cr+3)on DNA Damaging in Carp (Carassius auratus)Kidney Cells |
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| Zhao Shou Cheng Zhang Juan.Study on the Effects of Chromium (Cr+3)on DNA Damaging in Carp (Carassius auratus)Kidney Cells[J].Hebei Fisheries,1999(4):11-13. |
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| Authors: | Zhao Shou Cheng Zhang Juan |
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| Institution: | Dept.of Cell Biology .North-China Coal Medical College |
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| Abstract: | Using single cell gel - eletrophoresis (or comet assay)this paper presents the effects of chronium(Cr+3)on DNA breaks in.carp(Carassius auratus)kidney cells. The results show that a highly significant increase in DNA migration in DNA-damaged carp kidney cells was induced by three concentrations of chromium(Cr+3)compared with control (P< 0. 01). The percentage of DNA-damaged carp kidney cells was also increased. It is suggested that chromium can induce DNA damage and has toxic effect on carp kidney cells |
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| Keywords: | Chromium DNA damage Single cell gel -electrophoresis |
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