杭白芷种质资源遗传多样性的SSR分析

目的]分析杭白芷种质资源的遗传多样性,为其可持续利用及品种选育研究提供理论依据.方法]从已发表的18对白芷同属植物当归(Angelica sinensis)SSR引物中,筛选出6对具有较高多态性的引物,对3个杭白芷种群KZ (昆明)、NZ(南京)和XY(咸阳)]的53份种质材料进行PCR扩增,根据其基因型数据,分别采用Genepop 4.0、GenALEx 6.4.1和Structure 2.2进行遗传多样性、遗传差异及聚类分析.结果]6个SSR位点共扩增出37.0个等位基因,平均每个位点的等位基因数(Na)6.2个、有效等位基因数(Ne)1.798个,Shanon信息指数(I)1.802,观测杂合度(Ho)0.245,期望杂合度(He)0.304,基因流(Nm)为1.340.3个种群的遗传多样性存在一定差异,其中KZ种群遗传多样性最高(Na 3.8个,Ho 0.481,He 0.508).3个杭白芷种群可聚类分成两组,KZ种群独成一组,NZ种群和XY种群聚为一组,表明XY种群与NZ种群亲缘关系较近.XY种群与NZ种群间的遗传分化指数(FST)较低(0.208),表明二者遗传分化程度较低,遗传差异较小;KZ种群与NZ种群和XY种群间的FST均较高(0.510和0.506),表明KZ种群与NZ种群和XY种群遗传分化程度较高,遗传差异较大.结论]同属不同种的SSR引物具有一定通用性.不同来源地的杭白芷种群间存在一定程度的遗传分化,但总体遗传多样性较低,应加大力度寻找其近缘野生资源,保障白芷种质资源的可持续利用.

SSR analysis for genetic diversity of Angelica dahurica var. formosana

Objective]The genetic diversity of Angelica dahurica var.formosana gerplasm resources was analyzed to provide reference for sustainable utilization and new varieties breeding of A.dahurica gerplasm resources.Method]Six pairs of polymorphic SSR primers were screened from the published 18 pairs of SSR primers in A.sinensis,another An-gelica species.Fifty-three samples from three populationsKZ(Kunming),NZ(Nanjing),XY(Xianyang)]of A.dahurica were amplified by PCR.The genetic diversity,genetic difference and cluster analysis were conducted by GENEPOP 4.0, GenALeX 6.4.1 and Structure 2.2 respectively based on their genotype data.Result]In all six SSR loci,a total of 37 al-leles were amplified,the average allele number(Na)was 6.2,effective allele number(Ne)1.798,and Shannon informa-tion index(I)1.802.The observed heterozygosity(Ho),expected heterozygosity(He)and gene flow(Nm)were 0.245, 0.304 and 1.340,respectively.Furthermore,the genetic diversity among the three populations presented some differences. KZ population had the highest genetic diversity(Na 3.8,Ho 0.481 and He 0.508).The three populations were clustered in-to two groups:KZ population was in one group and NZ and XY populations were in another.It indicated that genetic rela-tionship between XY population and NZ population was close.The genetic difference analysis presented that,genetic dif-ferentiation index(FST)between XY population and NZ population was low(0.208),which indicated that genetic differen-tiation level between the two was low and genetic difference was small.FST between KZ and NZ populations(0.510),KZ and XY populations(0.506)were high,which meant the genetic differentiation levels for them were high and genetic di-fference was great.Conclusion]SSR primers of different species belong to the same genus may be universalizable. A.da-hurica populations from different places present certain genetic differentiation,but genetic diversity in general is low. Therefore,it is necessary to search closely related wild resources of A.dahurica to ensure the sustainable utilization of an-gelica root resources.