杉木优良无性系组培技术体系的建立

[目的]建立杉木(Cunninghamia lanceolata)优良无性系组培快繁技术体系,为杉木规模化育苗提供技术支持.[方法]以桂林市全州县15年生优良杉木单株基部或根部萌芽条的茎尖为外植体,筛选最佳HgCl2浸泡灭菌时间;以1/2MS为基本培养基添加不同激素组合进行芽的诱导和增殖,以1/4MS为基本培养基添加不同生长素或生长素组合进行生根培养,筛选适宜杉木组织培养和快繁的培养基.[结果]在改良培养基1/2MS+0.8 mg/L 6-苄氨基嘌呤(6-BA)+0.3 mg/L吲哚丁酸(IBA)中,杉木茎尖芽的诱导率达74.3%,平均芽长达2.3 cm;在改良培养基1/2MS+0.6 mg/L 6-BA+0.3 mg/L IBA中,杉木茎尖诱导芽的继代培养增殖倍数适中,增殖芽生长较快,有效苗数较多;在改良培养基1/4MS+0.5 mg/L IBA+1.0 mg/L ABT 6号生根粉中,杉木继代苗的生根率达90.7%.[结论]6-BA质量浓度是影响杉木外植体诱导率的主要因素,同时影响新芽的萌发数量;IBA则主要影响新芽的生长速度.适宜质量浓度的生长素可促进杉木组培苗生根,但质量浓度过高会抑制苗木生根.在实际生产中,以1/2MS+0.8 mg/L 6-BA+0.3 mg/L IBA为杉木茎尖芽诱导和生长的培养基、1/2MS+0.6 mg/L 6-BA+0.3 mg/L IBA为杉木茎尖诱导芽的继代增殖培养基、1/4MS+0.5 mg/L IBA+1.0 mg/L ABT 6号生根粉为杉木组培苗的生根培养基,可实现杉木优良无性系规模化育苗.

Establishment of tissue culture technique system on Cunninghamia lanceolata superior clone

[Objective]A Cunninghamia lanceolata superior clone rapid propagation technology system was estab-lished to provide technical for large-scale C. lanceolata seedling production.[Method]Using stem tip of stem base or root germination branch of 15-year C. lanceolata in Quanzhou,Guilin as the explants,the optimal HgCl2 soaking sterilization time was selected,and buds were induced and proliferated by adding different hormone combinations to 1/2MS as the ba-sic medium,and different auxins or auxin combinations were added to 1/4MS as the basic medium for rooting culture,in order to screen out the appropriate medium in tissue culture of C. lanceolata.[Result]The induction rate of bud of C. lan-ceolata stem tip was 74.3% and the average length of induced buds was 2.3 cm in culture medium of 1/2MS+0.8 mg/L 6-benzylaminopurine(6-BA)+0.3 mg/L indole butyric acid(IBA). In culture medium 1/2MS+0.6 mg/L 6-BA+0.3 mg/L IBA,the subculture multiplication number of the induced buds was moderate,the bud growth was fast and effective bac-teria number was large. Rooting rate of C. lanceolata subculture seedlings could be up to 90.7% in culture medium of 1/4MS+0.5 mg/L IBA+1.0 mg/L ABT No.6 rooting powder.[Conclusion]6-BA mass concentration is the main factor that affects induction rate of C. lanceolata explant,and also affects germination number of buds. IBA mainly affects growth speed of the buds. The proper mass concentration of auxin can promote rooting of C. lanceolata tissue culture seedlings, but high mass concentration auxin inhibits the rooting. In actual production,using 1/2MS+0.8 mg/L 6-BA+0.3 mg/L IBA as medium for induction and growth of bud of C. lanceolata stem tip,1/2MS+0.6 mg/L 6-BA+0.3 mg/L IBA as subcul-ture medium for induced bud of C. lanceolata stem tip and 1/4MS+0.5 mg/L IBA+1.0 mg/L ABT No.6 rooting powder as rooting medium for tissue culture seedling of C. lanceolata,large-scale C. lanceolata superior clone seedling production can be realized.