| 猪传染性胃肠炎病毒河北分离株核衣壳N蛋白基因的克隆与序列分析 |
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| 引用本文： | 张莉,翁崇鹏,毛娅卿,傅光华,张培君. 猪传染性胃肠炎病毒河北分离株核衣壳N蛋白基因的克隆与序列分析[J]. 中国兽药杂志, 2005, 39(6): 14-17 |
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| 作者姓名： | 张莉 翁崇鹏 毛娅卿 傅光华 张培君 |
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| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所,北京,100089
2. 江西农业大学动物科学技术学院,江西南昌,330045 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用RT-PCR技术扩增了TGEV河北分离株的N基因,长度约为1 149 bp. 将该基因片段进行亚克隆重组到pMD18-T质粒载体上,连接转化,鉴定后得到阳性重组质粒pTN.对重组质粒插入的目的基因片段进行序列分析和比较,结果表明TGEV河北分离株N基因与国外的Purdue-115、FS772/70、TO14、96-1933株有≥95%的同源性;推导的氨基酸序列同源性≥95%.TGEV N基因的克隆及序列分析,为其蛋白质的表达分析提供了重要依据,也为进一步讨论该病毒的分子生物学特性奠定了理论基础.
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| 关 键 词： | 猪传染性胃肠炎病毒 N基因 分子克隆 序列分析 |
| 文章编号： | 1002-1280(2005)06-0014-04 |
| 修稿时间： | 2004-04-20 |
Clone and sequence analysis of N gene from TGEV Hebei strain |
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| ZHANG Li,WENG Chong-peng,MAO Ya-qing,Fu Guang-hua,ZHANG Pei-jun. Clone and sequence analysis of N gene from TGEV Hebei strain[J]. Chinese Journal of Veterinary Drug, 2005, 39(6): 14-17 |
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| Authors: | ZHANG Li WENG Chong-peng MAO Ya-qing Fu Guang-hua ZHANG Pei-jun |
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| Abstract: | The viral subgenome mRNA of porcine transmissible gastroenteristis virus(TGEV) isolated from Hebei was amplified with RT-PCR. A DNA fragment about 1 149 bp was amplified. It contained the complete ORF of N gene. PCR products were purified and then cloned into plasmid vector PMD18-T. The recombinant plasmid was designated as pTN and analyzed by endonecleoase digestion from proper inserts. The sequence analysis of the insert fragment in the recombinant pTN indicated that TGEV isolated from Hebei shared more than 95% with Purdue-115, FS772/70, TO14 and 96-1933 strains. The amino acid sequence homology was not less than 95%. The clone and sequence analysis of N gene from TGEV provided a basis for the study of protein expressing and molecular characteristics. |
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| Keywords: | TGEV N gene clone sequence analysis |
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