环境胁迫对福寿螺肝胰脏抗氧化酶活性及丙二醛含量的影响

福寿螺（Pomacea canaliculate）是全球性的入侵物种，对入侵地的生物多样性、农业生产、人居环境及人体健康造成了重大危害，温度、盐度、溶解氧等环境因子影响福寿螺的生存、生长与繁殖。本文研究广州、上海、成都三地的福寿螺在不同温度、不同盐度、不同溶解氧环境胁迫下，肝胰脏超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量的变化情况，阐明环境胁迫对福寿螺生理的影响机理。实验结果表明：在温度、溶解氧、盐度单因子胁迫下，福寿螺肝胰脏的SOD活性、MDA含量均呈先上升后下降的变化趋势，且在24 h~48 h达到最大值，在72 h时恢复到初始水平。在温度-盐度复合胁迫下，福寿螺肝胰脏SOD活性及MDA含量高于单因素胁迫，温度和盐度表现出协同作用。单因素或复合胁迫下，福寿螺肝胰脏SOD活性及MDA含量存在地区差异。

Effects of environmental stress on the activities of antioxidant enzymes and malondialdehyde content in hepatopancreas of apple snails

Pomacea canaliculate is a global invasive species, which has caused great harm to the biological diversity, agricultural production, human settlements and human health of the invasive areas. Environmental factors such as temperature, salinity, dissolved oxygen affect the survival, growth and reproduction of Pomacea canaliculate. In this paper, the changes of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in hepatopancreas of Pomacea canaliculate in Guangzhou, Shanghai and Chengdu under different temperature, salinity and dissolved oxygen environmental stresses were studied to clarify the mechanism of environmental stress on the physiology of Pomacea canaliculate. The experimental results showed that under the stress of temperature, dissolved oxygen and salinity, the SOD activity and MDA content of the hepatopancreas of the snails increased first and then decreased, and reached the maximum value at 24 h~48 h, and recovered to the initial level at 72 h. Under the combined temperature-salinity stress, the SOD activity and MDA content in the hepatopancreas of the snails were higher than those under the single factor stress, and the temperature and salinity showed a synergistic effect. Under single or complex stress, SOD activity and MDA content in liver and pancreas of the snails varied from region to region.