| 用生长直径方法研究影响细菌活性物质提取效率的若干因素 |
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| 引用本文： | 谌晓曦. 用生长直径方法研究影响细菌活性物质提取效率的若干因素[J]. 上海交通大学学报(农业科学版), 2004, 22(1): 104-108 |
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| 作者姓名： | 谌晓曦 |
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| 作者单位： | 浙江中医学院,杭州,310053 |
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| 基金项目： | 浙江省医药卫生科学研究基金(2002A070)，浙江中医学院院内课题基金资助项目. |
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| 摘 要： | 建立了判断对357细菌活性物质相对浓度的方法即生长直径法，同时指出，抑菌圈不能有效地反映357细菌活性物质的相对浓度。生长直径法研究表明，不同培养基和不同培养时间可影响357细菌活性物质的分泌量，而pH值对用乙醚从水溶液中萃取活性物质的效率有较大影响。
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| 关 键 词： | 生长直径法 抑菌圈 培养基 培养时间 pH值 Bacillus cereus 357 |
| 文章编号： | 167-9964(2004)01-0104-05 |
| 修稿时间： | 2003-11-03 |
Effects of Several Factors on Isolation of Bioactive Substance Produced by Bacillus cereus 357 Using the Method Growth Diameter |
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| CHEN Xiao xi. Effects of Several Factors on Isolation of Bioactive Substance Produced by Bacillus cereus 357 Using the Method Growth Diameter[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2004, 22(1): 104-108 |
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| Authors: | CHEN Xiao xi |
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| Abstract: | A method used to estimate the concentration of the bio active substance produced by Bacillus cereus 357 has been established, Using this methods, several factors that effect secretion of the bioactive substance was studied. Meanwhile, for Bacillus cereus 357,the inhibition zone was not able to effectively determine the concentration of the bioactive substance. The growth diameter of fungi showed that Bacillus cereus 357 produced different amount of bioactive substance depending on the culture media as well as the culture duration. It also showed that the efficiency of extracting the bioactive substance from liquid containing such substance depended on the pH value. |
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| Keywords: | growth diameter inhibition zone culture media culture duration pH value Bacillus cereus 357 |
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