十三种淡水养殖鱼类的DNA含量

通过流式细胞仪测定了青鱼等十三种我国主要淡水养殖鱼类外周血红铁ＤＮＡ含量。其中青鱼、草鱼、鲢和鳙的ＤＮＡ含量比较接近，其２Ｃ值 ２．２０、２．１８、２．１８、２．１５ｐｇ，四种鱼相比较，差异不显著。

DNA content from thirteen species of cultured freshwater fishes in China

Flow cytometric DNA analysis of peripheral blood cells was used to determine the DNA contents from thirteen species of cultured freshwater fishes in China.The DNA contents of black carp (Mylopharyngodon piceus),glass carp(Ctenopharyngodon idellus),silver carp(Hypophthalmichthys molitrix) and bighead carp(Aristichthys nobilis) were 2.20,2.18,2.18 and 2.15pg/N respectively,which showed no significant difference between each two of them.Three common carps,Xinguo red carp(Cyprinus carpio var singuonesis),C.carpio with mirror carp and C.carpio with red colour,almost had the same content, 3.80,3.73 and 3.76pg/N respectively and no significant difference was observed.Two tilapia (Sarotherodon niloticus and S.aurea) also had the similar DNA content of 2.27pg/N and 2.22pg/N.The DNA contents from rainbow trout(Oncorhynchus mykiss),amur sturgeon(Acipenser schrensch) and blunt snout bream(Megalobrama amblycephala )were 3.47,11.73 and 2.66pg/N.respectively. Finally,the determination of systematic relationship of fish species from the DNA contents was suggested.