| 小金海棠Fe3+-还原酶基因MxFRO在酵母中的表达及其活性检测 |
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| 引用本文： | 任玲韩振海孔瑾许雪峰. 小金海棠Fe3+-还原酶基因MxFRO在酵母中的表达及其活性检测[J]. 农业生物技术学报, 2008, 16(5) |
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| 作者姓名： | 任玲韩振海孔瑾许雪峰 |
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| 作者单位： | 中国农业大学农学与生物技术学院园艺植物研究所 |
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| 摘 要： | 为研究苹果属植物抗缺铁的分子生物学机理，从铁高效基因型小金海棠（Malus xiaojinensis）中克隆了Fe3+-还原酶基因MxFRO。MxFRO2的cDNA序列长2283bp，开放阅读框为2166bp，编码722个氨基酸。半定量RT-PCR结果表明MxFRO在小金海棠的根和叶中均受缺铁胁迫诱导。 将MxFRO转入野生型酵母中，结果表明转基因酵母的Fe3+-还原酶活性是对照的2.8倍，因此我们初步推测MxFRO基因编码的蛋白具有Fe3+-还原酶活性。
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| 收稿时间： | 2007-04-25 |
| 修稿时间： | 2007-05-25 |
Expression and Ferric Reductase Activity of Ferric Reductase Gene MxFRO from Malus xiaojinensis in Transgenic yeast |
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| Abstract: | Malus xiaojinensis is iron-efficient genotype in the genus Malus. We isolated and cloned full-length cDNA sequence of MxFRO from Malus xiaojinensis , Semiquantitative RT-PCR was conducted, and MxFRO was found to be induced by iron-deficiency stress in roots and leaves. Yeast was transformed with MxFRO gene, and the ferric reductase activity in transgenic yeast was 2.8-fold that of controls.We concluded that MxFRO possessed ferric reductase activity. |
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