Humoral and cellular immune responses in dogs with inapparent natural Leishmania infantum infection |
| |
Authors: | Coura-Vital W Marques M J Giunchetti R C Teixeira-Carvalho A Moreira N D Vitoriano-Souza J Vieira P M Carneiro C M Corrêa-Oliveira R Martins-Filho O A Carneiro M Reis A B |
| |
Affiliation: | Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil. |
| |
Abstract: | Molecular analysis, serology and immunophenotyping for T lymphocytes and their subsets, B lymphocytes and monocytes were performed on dogs naturally infected with Leishmania infantum. Animals were categorised as asymptomatic dogs I (AD-I), with negative serology and positive molecular results, and asymptomatic dogs II (AD-II), with positive serology and positive molecular results, and these were compared to symptomatic dogs (SD) and control dogs (CD). AD-I exhibited immunophenotypic features similar to those of CD, including isotype profiles and concentrations of monocytes. Similar biomarkers were found in AD-II and SD, such as, higher levels of immunoglobulins IgG, IgG2, IgM and IgA and higher concentrations of eosinophils. High frequencies of T lymphocytes and CD4(+) T cells were observed in both AD-I and AD-II compared to SD, whereas CD8(+) T cells were higher only in AD-II compared with SD. Analysis of B lymphocytes revealed an increased frequency of this cell type only in AD-II animals compared with SD. Asymptomatic dogs appear to have a dichotomous infection spectrum that can influence the humoral and cellular immunological status during canine visceral leishmaniasis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|