加味大柴胡汤对阻塞性黄疸大鼠血浆内毒素的影响

目的：研究加味大柴胡汤保护阻塞性黄疸大鼠的肝功能、抑制脂质过氧化损伤及缓解内毒素血症的作用。方法：制作急性阻塞性黄疸大鼠模型，分为7，14及21d三个时段，每个时段分为3组，即：（1）假手术组（SO组）；（2）胆总管结扎＋生理盐水组（BDL＋NS组）；（3）胆总管结扎＋大柴胡汤（BDL＋中药组）。在各时相点，剖杀大鼠取肝组织及门静脉血，作病理检查；检测肝组织丙二醛（MDA）和门静脉内毒的含量。结果：（1）胆管结扎7、14、21d,BDL 中药组肝组织MDA含量显著高于SO组（P＜0．01），但低于BDL＋NS组（P＜0．01）；（2）胆管结扎7、14、21d后，门静脉血浆内毒素BDL＋NS组明显高于其它组（P＜0．01）。结论：大柴胡汤在提高机体抗病的能力、保护肝功能及减轻内毒素血症方面作用明显。

Effect of modified major bupleurum decoction on endotoxemia in rats with obstructive jaundice

Objective:To investigate the effect of modified major bupleurum decoction on liver function,lipid peroxidation and endotoxemia in rats with obstructive jaundice.Methods:Ninety SD rats were randomly divided into 3 groups:sham operation(SO)group( n =30),common bile duct ligation plus normal saline (BDL+NS)group( n =30),and common bile duct ligation plus major bupleurum decoction(BDL+MBD)group( n =30);acute obstructive jaundice models were established in the latter two groups.The rats were sacrificed on days 7,14 and 21,and the contents of MDA and endotoxin were determined.Results:MDA level in BDL+MBD group was higher than that in SO group( P< 0.01)and lower than that in BDL+NS group( P< 0.01)at 3 time points.Endotoxin concentrations of portal vein in BDL+NS group were superior to those in the other two groups ( P< 0.01)at each time point.Conclusion:Modified major bupleurum decoction could have the protection of liver function and alleviation of endotoxemia.