| 小麦苗期叶片中性蛋白水解酶酶谱的动态变化 |
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| 引用本文： | 姬生栋 夏民 等. 小麦苗期叶片中性蛋白水解酶酶谱的动态变化[J]. 麦类作物学报, 2001, 21(4): 80-82 |
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| 作者姓名： | 姬生栋 夏民 等 |
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| 作者单位： | 河南师范大学生命科学学院, |
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| 基金项目： | 河南省自然科学基金资助 ( 9740 1310 0 ) |
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| 摘 要： | 本试验采用复性电泳技术对小麦菌期蛋白水解酶种类及活性进行了动态研究。结果表明：（1）小麦从出苗第3d生长到第87d，153，72和68kD三条蛋白水解酶带均存在；（2）72和68kD两条蛋白水解酶带在各个时期均表现出很强的活性；（3）小麦的生理代谢影响着蛋白水解酶谱变化；（4）所检到的蛋白水解酶分子量均大于68kD(含68kD).
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| 关 键 词： | 小麦 蛋白水解酶 复性电泳 叶片 苗期 |
| 文章编号： | 1009-1041(2001)04-0080-03 |
| 修稿时间： | 2001-01-31 |
Dynamic Changes of Neutral Proteinase Enzymography in Wheat Leaf during Seedling Stage |
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| JI Sheng dong,XIA Min,LI Ji xue,WANG Dan,XU Cun shuan. Dynamic Changes of Neutral Proteinase Enzymography in Wheat Leaf during Seedling Stage[J]. Journal of Triticeae Crops, 2001, 21(4): 80-82 |
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| Authors: | JI Sheng dong XIA Min LI Ji xue WANG Dan XU Cun shuan |
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| Abstract: | We studied the types and dynamic changes of proteinase in wheat leaf during seedling stage by SDS G PAGE. The results showed that, (1) 153, 72 and 68 kDa proteinase bands were present from the 3rd to 87th days after the emergence of seedlings; (2) 72 and 68 kDa proteinase showed very strong activities throughout the seedling stage; and (3) the proteinase enzymography could be affected by the physiological metabolism. |
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| Keywords: | Wheat Proteinase SDS G PAGE |
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