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放线菌ECO 00008发酵液提取物抗稻瘟病的活性筛选研究
引用本文:尚慧,杨佩文,李明刚,刘树芳,李家瑞,文孟良. 放线菌ECO 00008发酵液提取物抗稻瘟病的活性筛选研究[J]. 西南农业学报, 2009, 22(5)
作者姓名:尚慧  杨佩文  李明刚  刘树芳  李家瑞  文孟良
作者单位:1. 云南省农业科学院农业环境资源研究所,云南,昆明,650205
2. 云南大学微生物研究所,云南,昆明,650091
基金项目:国家"十一五"科技支撑计划项目,云南省自然科学基金重点项目 
摘    要:从云南土壤中筛选分离到一株抗水稻稻瘟病菌的放线菌ECO 00008,对该菌株进行摇瓶发酵后采用乙酸乙酯萃取发酵液,测定其提取物(FR、FE、MR、ME)对水稻稻瘟病的抗菌活性.结果表明,萃取物FE和ME对水稻稻瘟病菌孢子萌发和菌丝生长具有较强的抑制活性,对孢子萌发抑制率分别为99.00 %和95.60 %,盆栽保护效果分别为97.61 %和75.07 %.

关 键 词:放线菌发酵液提取物  稻瘟病  抗菌活性

Study on Inhibitive Activity of Fermentation Extracts of Actinomycete ECO 00008 Against Pyricularia grisea
SHANG Hui,YANG Pei-wen,LI Ming-gang,LIU Shu-fang,LI Jia-rui,WEN Meng-liang. Study on Inhibitive Activity of Fermentation Extracts of Actinomycete ECO 00008 Against Pyricularia grisea[J]. Southwest China Journal of Agricultural Sciences, 2009, 22(5)
Authors:SHANG Hui  YANG Pei-wen  LI Ming-gang  LIU Shu-fang  LI Jia-rui  WEN Meng-liang
Abstract:One actinomycetes strain ECO 00008 against Pyricularia grisea was isolated from a soil sample collected from Yunnan. The extracts (FR,FE,MR,ME) of the actinomycete was carried out and the fermentation liquid was extracted with acetic ether. The inhibitive activity of the fermentation extracts against Pyricularia grisea were analyzed. The results showed that the fermentation extracts of FE and ME had significant inhibitive activity to mycelium growth and 99.00 %,95.60 % inhibitive activity rate to spore germination and the control efficiency of potted plant was 97.61 %,75.07 % against Pyricularia grisea.
Keywords:Fermentation extracts of actinomycete  Pyricularia grisea  Inhibitive activity
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