水稻小粒矮突变性状与T-DNA插入标签的共分离检测

 从T-DNA水稻标签系中发现1个标签系中出现了小粒矮(sgd)突变性状的分离。为克隆该突变基因和进行基因功能研究，对该突变体进行了遗传分析。该标签系存在多个插入事件，且T3代发现目标突变性状分离系中，另一个非目标插入事件已经纯合，故特异PCR检测不能确认小粒矮突变性状与T-NA插入共分离。但通过TAIL-CR方法，获得了插入点侧翼的水稻基因组序列，并证实该侧翼序列与小粒矮突变性状共分离。

Co-segregation Analysis Between T-DNA Tag and Character of Small Grain Dwarf Mutant in Rice

A small grain dwarf(sgd)mutant was identified in the segregated population from a T-DNA-tag rice line.Genetic analysis of this mutant was conducted for cloning and functional research of the gene.Multiple T-DNA insertion copies in this line were confirmed,and one of them had been homozygous in T3 segregation population.As a result,it was impossible to detect co-segregation between the tag and sgd merely depended on the specific PCR primer from T-DNA in T3 and successively generations.However,a flanking sequence from one of T-DNA insertion sites was obtained by TAIL-PCR,and was confirmed to be co-segregated with sgd in consecutively segregated generations.