| 瓠瓜种质资源遗传多样性的RAPD分析 |
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| 引用本文： | 高山,许端祥,林碧英,钟开勤.瓠瓜种质资源遗传多样性的RAPD分析[J].分子植物育种,2007,5(4):502-506. |
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| 作者姓名： | 高山 许端祥 林碧英 钟开勤 |
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| 作者单位： | 福州市蔬菜科学研究所,福州,350012;福建农林大学园艺学院,福州,350012 |
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| 摘 要： | 采用RAPD分子标记对源自中国7个省份的38份瓠瓜种质资源进行遗传多样性分析.20个随机引物共扩增出104条多态性带,平均每个引物扩增的多态性带数为5.2条,多态性位点百分率为51.2%.利用NTSYS-pc软件计算种质问的遗传距离,用15PGMA法进行聚类分析,将供试的38份种质可分为3个类群8组,用EIGEN方法进行主坐标分析,将其分为3个类群13组.两种分类方法所获结果基本一致.RAPD分子标记表现出与瓠瓜的农艺性状分类和地区分布有一定的相关性.
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| 关 键 词： | 瓠瓜 种质 遗传多样性 RAPD |
RAPD Analysis of Genetic Diversity on 38 Gelmplasm Resources of Lagenaria Siceraria |
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| Gao Shan,Xu Duanxiang,Lin Biying,Zhong Kaiqin.RAPD Analysis of Genetic Diversity on 38 Gelmplasm Resources of Lagenaria Siceraria[J].Molecular Plant Breeding,2007,5(4):502-506. |
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| Authors: | Gao Shan Xu Duanxiang Lin Biying Zhong Kaiqin |
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| Institution: | 1 Fuzhou Institute of Vegetable Crops, Fuzhou, 350012; 2 College of Horticulture in Fujian Agriculture and Forestry University, Fuzhou, 350012 |
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| Abstract: | RPAD markers were used to detect the genetic diversity among 38 Bottle Gourd accessions from 7 provinces in China. Twenty RAPD random primers produced 104 polymorphic bands, averaged 5.2 bands each primer pair. The average percentage of polymorphic bands was 51.2%. Genetic distance was calculated by the NTSYS-pc software. A dendrogram was constructed using UPGMA based on genetic distance matrix. 38 accessions were clustered into 3 groups and 8 subgroups based on the RAPD data by the method of clustering analysis, and 3 groups and 13 subgroups by the method of principal coordinates analysis. The two methods above exhibited similar phylogenies among the accessions. The results from RAPD molecular markers showed obvious correlation with the agronomic characteristics classification and the locations distribution of the Bottle Gourd accessions. |
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| Keywords: | Lagenaria siceraria (Mol ) Stand Germplasm Genetic diversity RAPD |
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