| 水蛭素的全基因合成及克隆 |
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| 引用本文： | 许长蔼,张要武,向开军,李爱华. 水蛭素的全基因合成及克隆[J]. 华北农学报, 2000, 15(4): 58-61 |
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| 作者姓名： | 许长蔼 张要武 向开军 李爱华 |
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| 作者单位： | 天津市农业生物工程研究中心,天津,300192 |
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| 摘 要： | 报道了水蛭素全基因的合成及克隆。根据已知水蛭素氨基酸序列及酵母菌体内蛋白质表达特性,设计了水蛭素的全基因序列,采用化学合成及酶学相结合的方法,合成了该基因片段,并逐段克隆至载体ｐＢＳ上,得到全长水蛭素基因。序列测定表明,克隆后ＤＮＡ顺序同原设计序列。
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| 关 键 词： | 水蛭素 基因合成 基因克隆 基因重组 抗凝血药物 |
| 修稿时间： | 1999-01-27 |
Sythesis and Cloning of Hirudin Gene |
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| XU Chang-ai,ZHANG Yao-wu,XIANG Kai-jun,LI Ai-hua. Sythesis and Cloning of Hirudin Gene[J]. Acta Agriculturae Boreali-Sinica, 2000, 15(4): 58-61 |
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| Authors: | XU Chang-ai ZHANG Yao-wu XIANG Kai-jun LI Ai-hua |
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| Abstract: | A 240-bp DNA fragment coding for the hirudin was chemically and enzymically sythesized.The sequence was derived from the amino acid sequence of the hirudin peptide,which is consistent with the request of yeast gene expression.The sythesis involved preparation of six long oligodeoxyribonucleotides.After annealing,filling by Klenow fragment,three formed fragments were cloned into pBS sequentially,and an intact hirudin gene was obtained.The DNA sequence of the cloned fragment identified by sequencing was the same as the designed sequence of hirudin gene |
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| Keywords: | Hirudin Gene Sythesis Cloning |
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