| 矿物油室温保存棉花黄萎菌的ISSR分析 |
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| 引用本文： | 杨继娟,张吉光,胡小平,朱荷琴,杨家荣. 矿物油室温保存棉花黄萎菌的ISSR分析[J]. 西北农业学报, 2010, 19(12): 177-182 |
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| 作者姓名： | 杨继娟 张吉光 胡小平 朱荷琴 杨家荣 |
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| 作者单位： | 1. 西北农林科技大学,植保学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100
2. 中国农业科学院,棉花研究所,河南安阳,455000 |
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| 基金项目： | 教育部新世纪优秀人才支持计划(NCET-07-0698); 陕西省自然科学基金(2010JM3018); 西北农林科技大学科研专项基金资助(QN2009034) |
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| 摘 要： | 从ITS序列和ISSR分子标记水平对45株棉花黄萎菌进行遗传变异研究,共扩增到178条多态性条带,呈现丰富的多态性。依据ISSR分子标记的聚类分析结果、菌株的致病性以及ITS测序结果,可将45个菌株分为弱致病类型大丽轮枝菌、强致病类型大丽轮枝菌和变黑轮枝菌3类,ISSR分子标记多态性与棉花黄萎病菌的致病类型呈现一定的相关性,但与保存年限间无明显的相关性,所有菌株均表现出了典型的分子标记模式,仅有菌株394的ITS序列出现了单位点突变。
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| 关 键 词： | 棉花黄萎病菌 ITS ISSR 矿物油保存 致病性 |
ISSR Analysis of Verticillium Wilt Pathogen Maintained in Mineral Oil at Room Temperature |
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| YANG Jijuan,ZHANG Jiguang,HU Xiaoping,ZHU Heqin and YANG Jiarong. ISSR Analysis of Verticillium Wilt Pathogen Maintained in Mineral Oil at Room Temperature[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(12): 177-182 |
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| Authors: | YANG Jijuan ZHANG Jiguang HU Xiaoping ZHU Heqin YANG Jiarong |
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| Affiliation: | YANG Jijuan1,ZHANG Jiguang1,HU Xiaoping1,ZHU Heqin2 and YANG Jiarong1(1.College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest A&F University,Yangling Shaanxi 712100,China,2.Cotton Research Institute,The Chinese Academy of Agricultural Sciences(CAAS),Anyang Henan 455000,China) |
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| Abstract: | The genetic variation analysis of 45 isolates of Verticillium wilt pathogen was conducted through ITS(internal transcribed spacer) sequences and ISSR(internal simple sequence repeat) markers.One hundred and seventy-eight polymorphism bands were obtained from ISSR markers.Based on the clustering result of ISSR marker data,pathogenicity and ITS sequence of the strains,forty five isolates were divided into three groups: V.dahliea with weak virulence,V.dahliea with strong virulence,and V.nigrescens.The polymorp... |
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| Keywords: | Verticillium dahliae Internal transcribed spacer(ITS) Internal simple sequence repeat(ISSR) Mineral oil preservation Pathogenicity |
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