J亚群禽白血病病毒(ALV-J)ELISA检测方法的建立

利用抗J亚群禽白血病病毒（ALV—J）囊膜蛋白特异性单克隆抗体JE9，建立了检测ALV—J env抗原抗体免疫复合物的ELISA方法。应用该方法检测SPF鸡血清、鸡抗禽流感H9亚型阳性血清、鸡沙门氏菌阳性血清、鸡腺病毒阳性血清、鸡新城疫阳性血清．结果均为阴性，无交叉反应；抗ALV—J阳性血清与ALV—J特异性单克隆抗体JE9能相互阻断；ALV—J阳性血清经酸处理后，ELISA检测的D490差值明显下降。对部分攻毒鸡血清样本及田间ALV—J阳性血清样本进行电镜观察，可见ALV—J样病毒粒子及ALV—J样免疫复合物。经与间接免疫荧光（IFA）检测ALV—J env抗体结果比较表明，建立的ELISA方法与IFA方法两者具有较好的群体符合率，群体符合率为8／9。这些结果表明，本研究建立的ELISA方法在ALV—J的诊断中具有很好的应用前景。

Establishment of ELISA for Diagnosis of Subgroup J of Avian Leukosis Virus(ALV-J)

An ELISA procedure to detect subgroup J of avian leucosis virus(ALV-J) was established with monoclonal antibody JE9 specific to envolop protein of ALV-J.ALV-J infection could be diagnosed by the ELISA through detecting the antigen-antibody complex in serum.There was no cross-reaction with SPF chicken sera or sera to avian influenza virus,avian adenovirus,Newcastle disease virus or Salmonella.The positive serum to ALV-J and the monoclonal antibody JE9 could block with each other in the ELISA.ALV-J-like virons and immuno-complex were observed in the positive chicken serum under electronic microscopy.The index of ELISA for positive serum rapidly decreased after treatment with low pH.Comparing with immunofluorenscence assay(IFA) results,both mothods showed a good compatibility in the flocks of chickens.These results indicated that this ELISA will be useful in the ALV-J control in future.