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Plant regeneration in <Emphasis Type="Italic">Actinidia polygama</Emphasis> Miq. by leaf,stem, and petiole culture with zeatin,and from stem-derived calli on low-sucrose medium
Authors:Wataru?Takahashi  Fuyuki?Sugawara  Noriko?Yamamoto  Eriko?Bando  Junjo?Matsushita  Email author" target="_blank">Osamu?TanakaEmail author
Institution:(1) Department of Biology, Faculty of Science and Engineering, Konan University, Kobe, 658-8501, Japan;(2) Akita Prefecture Forest Technical Center, Akita, Japan
Abstract:We examined the effect of zeatin on the formation of shoot buds from explants and callus tissues derived from stem segments of Actinidia polygama Miq. (matatabi or silver vine). Stem and petiole segments cultured on combined broad-leaved tree medium and woody plant medium (BW medium) supplemented with zeatin for 40 days formed many shoot buds. Callus tissues derived from the stem segments and subcultured on BW medium supplemented with 6-benzyladenine and 1-naphthaleneacetic acid formed shoot buds when the medium contained 13.7thinspµM zeatin. BW medium containing low concentrations of sucrose strongly induced the formation of shoot buds from the callus.
Keywords:Actinidia polygama Miq    Callus  Plant regeneration  Sucrose  Zeatin
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