| 简便高效的蛋白质双相氯胺-T碘标法的建立及其应用 |
| |
| 引用本文： | 包伦,汪淑芳,徐贤坤,王霜,曾宪垠.简便高效的蛋白质双相氯胺-T碘标法的建立及其应用[J].四川农业大学学报,2005,23(4):428-431. |
| |
| 作者姓名： | 包伦 汪淑芳 徐贤坤 王霜 曾宪垠 |
| |
| 作者单位： | 四川农业大学,生命科学与理学院,四川,雅安,625014;四川农业大学,生命科学与理学院,四川,雅安,625014;四川农业大学,生命科学与理学院,四川,雅安,625014;四川农业大学,生命科学与理学院,四川,雅安,625014;四川农业大学,生命科学与理学院,四川,雅安,625014 |
| |
| 摘 要： | 对双相氯胺-T碘标法实验装置作了改进并标记长效促红细胞生成素(LL-EPO),采用离心超滤技术代替传统的柱层析脱盐纯化标记蛋白质。结果显示125I-LL-EPO具有高比活性、高生物活性、高回收率。说明双相氯胺-T碘标法简单方便,是蛋白质同位素碘标记的理想方法;离心超滤技术适合放射性碘标记蛋白质的纯化。
|
| 关 键 词： | 同位素碘标记 双相氯胺-T LL-EPO 离心超滤 |
| 文章编号： | 1000-2650(2005)04-0428-04 |
| 收稿时间： | 05 27 2005 12:00AM |
| 修稿时间： | 2005年5月27日 |
Establishment of a Convenient and High Efficient Method for Two-phase Chloramine-T Iodination and Applying to Protein |
| |
| BAO Lun,WANG Shu-fang,XU Xian-kun,WANG Shuang,ZENG Xian-yin.Establishment of a Convenient and High Efficient Method for Two-phase Chloramine-T Iodination and Applying to Protein[J].Journal of Sichuan Agricultural University,2005,23(4):428-431. |
| |
| Authors: | BAO Lun WANG Shu-fang XU Xian-kun WANG Shuang ZENG Xian-yin |
| |
| Institution: | College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China |
| |
| Abstract: | This research presented a modified two-phase chloramine-T method for radioiodine labeling of protein and Long-lasting Erythropoietin(LL-EPO) was labeled.And traditional gel filtration was substituted by ultrafiltration to purify the labeled protein.The results show that labeled protein possesses high specific radioactivity,biological activity and recovery.It suggests that two-phase chloramine-T method is simple and convenient and an ideal method to iodinate protein.Ultrafiltration is suitable for purification of the radioiodine labeled protein. |
| |
| Keywords: | radioiodine labeling two-phase chloramine- T LL- EPO ultrafiltration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
