| 香蕉ACC合成酶cDNA5‘末端的快速扩增 |
| |
| 引用本文： | 金志强,徐碧玉,邵寒霜,彭世清,郑学勤.香蕉ACC合成酶cDNA5‘末端的快速扩增[J].热带作物学报,2001,22(3):24-28. |
| |
| 作者姓名： | 金志强 徐碧玉 邵寒霜 彭世清 郑学勤 |
| |
| 作者单位： | 1. 中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101
2. 华南热带农业大学园艺学院,海南,儋州,571737 |
| |
| 基金项目： | 国家自然科学基金资助项目(批准号:39460053) |
| |
| 摘 要： | 采用cDNA末端快速扩增（Rapid amplification of cDNA ends,RACE）的方法对香蕉ACC合成酶的cDNA5'末端进行了扩增，获得677bp的产物，序列测定的结果表明，其中一个连续编码149个氨基酸，其序列含有ACC合成酶的第一和第二保守区和一段长230bp的非翻译区。
|
| 关 键 词： | 香蕉 ACC合成酶 cDNA5‘ 末端扩增 |
| 修稿时间： | 2000年6月9日 |
Rapid Amplification of cDNA with 5'end Encoding ACC Synthase in Banana (Musa AAA Cavendish Subgroup) |
| |
| Jin Zhiqiang,Xu Biyu,Shao Hanshuang,Peng Shiqing,Zheng Xueqin.Rapid Amplification of cDNA with 5''''end Encoding ACC Synthase in Banana (Musa AAA Cavendish Subgroup)[J].Chinese Journal of Tropical Crops,2001,22(3):24-28. |
| |
| Authors: | Jin Zhiqiang Xu Biyu Shao Hanshuang Peng Shiqing Zheng Xueqin |
| |
| Institution: | Jin Zhiqiang1 Xu Biyu2 Shao Hanshuang1 Peng Shiqing1 Zheng Xueqin1 |
| |
| Abstract: | A fragment of cDNA with 5' end encoding ACC synthase in banana (Musa AAA Cavendish Subgroup) was obtained by using rapid amplification of cDNA ends (RACE). The product of 5' RACE was 677 bp long and its sequencing showed that the longest encoding region contained 149 amino acids whose sequence contained the first and second highly conserved regions and 230 bp non-translated region of ACC synthase. |
| |
| Keywords: | Banana (Musa AAA Cavendish Subgroup) ACC synthase cDNA 5'RACE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
