| 反相高效液相色谱法分离中华绒螯蟹性腺抑制激素的初步研究 |
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| 引用本文： | 马奎,康现江,穆淑梅,张亮,温秀荣. 反相高效液相色谱法分离中华绒螯蟹性腺抑制激素的初步研究[J]. 中国水产科学, 2007, 14(2): 331-335 |
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| 作者姓名： | 马奎 康现江 穆淑梅 张亮 温秀荣 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省自然科学基金;河北大学青年科学基金 |
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| 摘 要： | 采用反相高效液相色谱层析法(RP-HPLC)分离中华绒螯蟹(Eriocheir sinensis)眼柄视上神经节中的性腺抑制激素,收集在高血糖激素家族区域内出峰的洗脱液组分,得到7组单一的样品组分。通过活体注射的方法,观察和统计每一组分对去眼柄中华绒螯蟹卵母细胞直径大小的影响,以检验每个分离组分的性腺抑制活性。结果发现,其中的第7组分对中华绒螯蟹的卵巢发育具有最强的抑制作用,推测这一组分为中华绒螯蟹的性腺抑制激素。
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| 关 键 词： | 中华绒螯蟹 性腺抑制激素 反相高效液相色谱 卵母细胞 |
| 文章编号： | 1005-8737-（2007）02-0331-05 |
| 收稿时间： | 2006-07-31 |
| 修稿时间： | 2006-10-23 |
Primary study of GIH isolation in Eriocheir sinensis by RP-HPLP |
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| MA Kui,KANG Xian-jiang,MU Shu-mei,ZHANG Liang,WEN Xiu-rong. Primary study of GIH isolation in Eriocheir sinensis by RP-HPLP[J]. Journal of Fishery Sciences of China, 2007, 14(2): 331-335 |
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| Authors: | MA Kui KANG Xian-jiang MU Shu-mei ZHANG Liang WEN Xiu-rong |
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| Affiliation: | College of Life Science, Hebei University, Baoding 071002, China |
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| Abstract: | The gonad inhibiting hormone(GIH) is a member of Crustacean hyperglycemic hormone(CHH) peptide family.It plays a significant role in inhibiting development of crab's gonad.In this research,the crude hormone extract of crab's optic ganglia was further isolated by reverse-phase high-performance liquid chromatography(RP-HPLC).Seven single fractions were obtained.The biological activity of each fraction was assayed through injecting in vivo to the crabs whose optic ganglia were removed.After 6 days of injection the diameter of crab's oocytes was recorded and statistically analyzed.The results showed that among the 7 isolated fractions,fraction 7 had the greatest biological activity to suppress the development of crab oocytes,which indicated that this fraction was the GIH of Eriooheir sinensis. |
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| Keywords: | Eriocheir sinensis gonad inhibiting hormone RP-HPLC oocyte |
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