| 根癌农杆菌介导茶树转化研究 |
| |
| 引用本文： | 赵东,刘祖生,陆建良,钱利生,屠幼英,奚彪.根癌农杆菌介导茶树转化研究[J].茶叶科学,2001,21(2):108-111. |
| |
| 作者姓名： | 赵东 刘祖生 陆建良 钱利生 屠幼英 奚彪 |
| |
| 作者单位： | 浙江大学农学院茶学系,浙江大学农学院茶学系,浙江大学农学院茶学系,浙江大学农学院茶学系,浙江大学农学院茶学系,浙江大学农学院茶学系 浙江杭州310029 ,浙江杭州310029 ,浙江杭州310029 ,浙江杭州310029 ,浙江杭州310029 ,浙江杭州310029 |
| |
| 基金项目： | 国家自然科学基金资助项目 (39770 4 75) |
| |
| 摘 要： | 通过检测GUS瞬间表达 ,对影响根癌农杆菌侵染茶树叶片效果的若干因素进行研究。Kan对茶树叶片愈伤组织形成的抑制浓度在 3 0mg/L左右 ,因而对以Kan为筛选标记的载体来说 ,5 0mg/LKan是较为适宜的选择浓度。茶树较为适宜的农杆菌转化系统是用OD6 0 0 为 0 5 - 0 8的LBA4 4 0 4侵染 10分钟左右 ,暗中2 8℃共培养 2 - 3天。硝酸银对农杆菌生长有抑制作用 ,不宜在共培养阶段添加
|
| 关 键 词： | 茶树 根癌农杆菌 转化 |
| 文章编号： | 1000-369X(2001)02-0108-04 |
| 修稿时间： | 2001年2月28日 |
Study on Agrobacterium tumefaciens-mediated Transformation of Tea Plant |
| |
| ZHAO Dong,LIU Zu_sheng,LU Jian_liang,QIAN Li_sheng,TU You_ying,XI Biao.Study on Agrobacterium tumefaciens-mediated Transformation of Tea Plant[J].Journal of Tea Science,2001,21(2):108-111. |
| |
| Authors: | ZHAO Dong LIU Zu_sheng LU Jian_liang QIAN Li_sheng TU You_ying XI Biao |
| |
| Abstract: | Some factors which affect the result of Agrobacterium tumefaciens infection leaf of tea plant (Camellia sinensis) was studied based on GUS transient expression 50 mg/L Kanamycin is suitable selection concentration for expression vectors with neomycin phosphotransferase gene (NPTⅡ) as callus formation from tea plant leaf was depressed by about 30 mg/L Kanamycin The suitable system of Agrobacterium tumefaciens _mediated transformation is suggested that tea plant leaf was infected 10 min by LBA4404 of OD600 about 0 5-0 8 followed by co_culture of 2-3 days about 28℃ in darkness. AgNO3 may inhibit the growth of Agrobacterium tumefaciens, so is not suitable to be applied into co-culture medium. |
| |
| Keywords: | Camellia sinensis Agrobacterium tumefaciens Transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《茶叶科学》浏览原始摘要信息 |
| 点击此处可从《茶叶科学》下载免费的PDF全文 |
|
