冬凤兰非共生萌发和低温离体保存

以冬凤兰成熟种子无菌萌发得到的原球茎为材料，建立冬凤兰原球茎低温离体保存技术体系，为冬凤兰的种质资源保存提供依据。

Asymbiotic Germination and Low-temperature in Vitro Conservation of Cymbidium Dayanum

[Objective]The aim of the research was to establish asymbiotic gemination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.[Method]With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorn~ as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.[Result]Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as"Hyponex 1"media.The germination rate reached more than 98%.Protocorms inoculated in"Hyponex 1"media could be conserved continuously at 5℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and"Hyponex 1"media.The seedling-strengthening and rooting media were 1/2 MS media.[Conclusion]This research provided practical basis for in votro conservation and rapid propagation of C.dayanum germplasm resource.