首页 | 本学科首页   官方微博 | 高级检索  
     

水牛胎儿卵泡细胞和卵母细胞凋亡的特征
引用本文:何宝祥,郑喜邦,杜玉兰,李彩萍,李恭贺,余四九. 水牛胎儿卵泡细胞和卵母细胞凋亡的特征[J]. 畜牧兽医学报, 2006, 37(1): 23-27
作者姓名:何宝祥  郑喜邦  杜玉兰  李彩萍  李恭贺  余四九
作者单位:1. 广西大学动物科学技术学院,南宁,530005
2. 甘肃农业大学动物医学院,兰州,730070
基金项目:广西科学基金项目(桂基科0144009);广西“新世纪十百千人才工程”专项资金(2001210)
摘    要:选择5月龄胎儿水牛的卵巢,采用石蜡切片、HE染色的光镜观察和DNA末端原位标记技术(TUNEL)进行了研究。结果表明:5月龄胎儿卵巢上分布着大量的凋亡细胞;在皮质部存在的大量腔前卵泡中,均匀分布有单个凋亡的卵泡细胞和卵母细胞,凋亡细胞的主要组织学变化是细胞缩小,核浓缩;DNA末端标记变化主要显示出TUNEL阳性。上述结果提示:水牛卵泡细胞和卵母细胞凋亡在胎儿时期已经存在,细胞凋亡是启动水牛腔前卵泡闭锁的潜在机理。

关 键 词:水牛胎儿 卵巢 细胞凋亡 卵母细胞 卵泡细胞
文章编号:0366-6964(2006)01-0023-05
收稿时间:2005-03-28
修稿时间:2005-03-28

The Apoptotic Characteristics of Follicular Cells and Oocytes in Buffalo Fetal Ovary
HE Bao-xiang,ZHENG Xi-bang,DU Yu-lan,LI Cai-ping,LI Gong-he,YU Si-jiu. The Apoptotic Characteristics of Follicular Cells and Oocytes in Buffalo Fetal Ovary[J]. Chinese Journal of Animal and Veterinary Sciences, 2006, 37(1): 23-27
Authors:HE Bao-xiang  ZHENG Xi-bang  DU Yu-lan  LI Cai-ping  LI Gong-he  YU Si-jiu
Affiliation:1. College of Animal Scientific and Technology , Guangxi University, Nanning 530005, China; 2. College of Animal Medicine, Gansu Agricultural University, Lanzhou 730070, China
Abstract:In order to understand if the apoptosis of follicular cells and oocytes exists in the buffalo fetals ovary,and if does,what is the apoptotic characteristics,ovaries was chosen from a five-month-old fetal buffalo,the apoptotic characteristics of follicular cells and oocytes was studied by paraffin section,HE staining,light microscope and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) technology.The results were shown that large number of apoptotic cells existed diffusedly on 5-month-old fetal ovary.Many apoptotic follicular cells and some apoptotic oocytes were distributed solely and evenly among a lot of preantrum follicles of(ovary) cortex. The main changes of apoptosis were cell shrinkage,nuclear condensed in histology,DNA terminal labeling appeared TUNEL positive.These results suggested that the apoptosis of(ovarian) follicular cells and oocytes was already existed in buffalo fetal ovary,and the apoptosis was the potential mechanism of arosing preantrum follicle atresia.
Keywords:buffalo fetal   ovary   apoptosis   oocyte   follicular cells
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号