Sulfated polysaccharides in marine sponges: extraction methods and anti-HIV activity |
| |
Authors: | Esteves Ana I S Nicolai Marisa Humanes Madalena Goncalves Joao |
| |
Institution: | Centre of Marine Sciences, University of Algarve, Gambelas Campus, 8005-139 Faro, Portugal. aidsesteves@gmail.com |
| |
Abstract: | The extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus, Cliona celata and Stelletta sp., collected in the Northeastern Atlantic, is presented in this work. The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. Crude extracts prepared from Erylus discophorus specimens were all highly active against HIV-1 (90 to 95% inhibition). Cliona celata pellets showed low polysaccharide content (bellow 38.5%) and almost no anti-HIV activity (<10% inhibition). Stelletta sp. pellets, although quite rich in polysaccharide (up to 97.3%), showed only modest bioactivity (<36% HIV-1 inhibition). Erylus discophorus pellets were among the richest in terms of polysaccharide content (up to 98%) and the most active against HIV-1 (up to 95% inhibition). Chromatographic fractionation of the polysaccharide pellet obtained from a specimen of Erylus discophorus (B161) yielded only modestly active fractions. However, we could infer that the active molecule is most probably a high molecular weight sulfated polysaccharide (>2000 kDa), whose mechanism is possibly preventing viral attachment and entry (fusion inhibitor). |
| |
Keywords: | porifera Erylus discophorus polysaccharides anti-HIV activity |
本文献已被 PubMed 等数据库收录! |
|