The effects of glibenclamide,a blocker of K+ ATP-sensitive potassium channels,on diaphragmatic fatigue during endotoxaemia in pigs |
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Authors: | G Aguggini M Dimori G Vanelli M Albertini |
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Institution: | (1) Institute of Veterinary Physiology and Biochemistry, University of Milan, Via Celoria 10, 20133 Milan, Italy |
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Abstract: | An in vivo porcine model of endotoxaemia was used to study the effects of glibenclamide, a K+ ATP-sensitive potassium channel blocker. Escherichia coli lipopolysaccharides (LPS, 70 g/kg, i.v., as a bolus) were infused into anaesthetized, mechanically ventilated, indomethacin-treated pigs. After 120 min of endotoxaemia, glibenclamide was administered (10 mg/kg, i.v., over 5 min) to half the pigs. The steength at different frequencies of stimulation (10, 20, 30, 50 Hz, 20 V, 1 s) and the endurance capacity (10 Hz, 20 V, 30 s) of the diaphragm were evaluated after 120 min of endotoxaemia and 5, 10, 20 and 30 min after drug infusion. Glibenclamide transiently increased the blood pressure without changing the decreased cardiac output and at the same time further impaired the diaphragmatic activity. The reduced ability of the diaphragm to generate force in response to different electrical stimulations was shown by a significant reduction in strength. The endurance index decreased 5 min after glibenclamide infusion, returning to the pre-glibenclamide values by 150 min. These results indicate that glibenclamide modifies the activity of vascular smooth muscle and of the diaphragm.Abbreviations BP
blood pressure
- CO
cardiac output
- LPS
lipopolysaccharides
- Glib
glibenclamide
- i.m.
intramuscular
- i.v.
intravenous
- SEM
standard error of the mean
- v/v
volume/volume P
ab, abdominal pressure
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P
di
transdiaphragmatic pressure
-
P
oes
oesophageal pressure |
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Keywords: | diaphragm endotoxaemia Escherichia coli fatigue glibenclamide potassium channel shock |
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