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大白菜-结球甘蓝单体异附加系试管苗生根能力的研究
引用本文:张巍巍,李晓峰,罗双霞,赵玉靖,王彦华.大白菜-结球甘蓝单体异附加系试管苗生根能力的研究[J].中国蔬菜,2011,1(6):28-32.
作者姓名:张巍巍  李晓峰  罗双霞  赵玉靖  王彦华
作者单位:1.河北农业大学园艺学院,河北保定 071000;2河北省科技工程学校,河北保定 071000
摘    要:以大白菜-结球甘蓝系列单体异附加系试管苗为试材,比较了不同单体异附加系试管苗的生根率和移栽成活率,研究了不同浓度NAA对单体异附加系试管苗生根和根尖细胞分裂的影响。结果表明:AA+C1、AA+C2、AA+C3、AA+C4、AA+C5和AA+C6的试管苗生根较好,移栽成活率高,AA+C7、AA+C8和AA+C9的试管苗不易生根,移栽成活率低;1/2MS培养基附加1.5 mg?mL-1 NAA能有效诱导试管苗生根,提高试管苗生根质量和移栽成活率。

关 键 词:大白菜  结球甘蓝  单体异附加系  试管苗  诱导生根  细胞分裂  
收稿时间:2010-11-23;

Studies on Plantlet Rooting of Chinese Cabbage-Cabbage Monosomic Alien Addition Lines
ZHANG Wei-wei,LI Xiao-feng,LUO Shuang-xia,ZHAO Yu-jing,WANG Yan-hua.Studies on Plantlet Rooting of Chinese Cabbage-Cabbage Monosomic Alien Addition Lines[J].China Vegetables,2011,1(6):28-32.
Authors:ZHANG Wei-wei  LI Xiao-feng  LUO Shuang-xia  ZHAO Yu-jing  WANG Yan-hua
Institution:1.College of Horticulture, Hebei Agricultural University, Baoding 071000, Hebei, China;2.Science and Engineering College of Hebei Province, Baoding 071000, Hebei, China
Abstract:With the plantlets of Chinese cabbage〔Brassica compestris L. ssp. pekinensis(Lour)Olsson〕-cabbage(Brassica oleracea L. var. capitata L.)monosomic alien addition lines as materials, comparison of rooting rate and transplanting survival rate among different monosomic alien addition lines and effect of NAA content in 1/2 MS medium on the root induction were conducted in this paper. The result showed that monosomic alien addition lines AA+C1, AA+C2, AA+C3, AA+C4, AA+C5, and AA+C6 had higher rooting and transplanting survival rate, whereas the rate were much lower for the AA+C7, AA+C8 and AA+C9, adding 1.5 mg?L-1 NAA in 1/2 MS could effectively induce the root initiation and raise the transplanting survival frequency of monosomic alien addition lines AA+C7, AA+C8 and AA+C9.
Keywords:Chinese cabbage  Cabbage  Monosomic alien addition line  Plantlet  Root induction  Cell division  
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