| 关于牛CD34cDNA的克隆及应用 |
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| 引用本文： | 朱沙,崔保安,陈圣先. 关于牛CD34cDNA的克隆及应用[J]. 中国畜牧兽医, 2002, 29(5): 35-36 |
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| 作者姓名： | 朱沙 崔保安 陈圣先 |
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| 作者单位： | 1. 河南农业大学牛业工程研究所,郑州,450002
2. 郑州兽药厂研究所,郑州,450008 |
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| 摘 要： | CD34是白细胞抗原，可在不同类型的细胞包括造血细胞中进行表达，抗人，鼠和犬CD34鼠白的单克隆抗体已用于对淋巴造血干细胞的鉴别，本试验克隆出编码牛CD34的cDNA，并测定其核苷酸顺序，推测其蛋白的氨基酸顺序与人，鼠和犬的CD34蛋白的氨基酸顺序的同源性分别为61.1%,56.0%,和66.1%。并以cDNA作为探针进行Northern杂交，探测CD34RNA在胎牛脑，脾，心和肺中的表达。
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| 关 键 词： | 牛 CD34cDNA 克隆 应用 |
| 文章编号： | 1671-7236(2002)05-0035-02 |
| 修稿时间： | 2002-03-26 |
Practicing and Cloning of Bovine CD34 cDNA |
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| ZHU Sha et al. Practicing and Cloning of Bovine CD34 cDNA[J]. China Animal Husbandry & Veterinary Medicine, 2002, 29(5): 35-36 |
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| Authors: | ZHU Sha et al |
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| Abstract: | CD34 is a leukocyte antigen that is expressed in various cell types including hematopoietic cell. Monoclonal antibodies against human murine and canine CD34 proteins have been used for the identification of lymphohematopoietic stem cell. The cDNA encoding bovine CD34 was determined. The identity of the deduced amino acid sequence of the encoded protein to those of human,murine and canine CD34 proteins was 61.1%,56.0%and 66.1% respectively. Northern blot hybridization with the cDNA as a probe dctected CD34 cRNA expression in the cerebrum,spleen,heart and lung of a fetal calf. |
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| Keywords: | Boine CD34 cDNA |
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