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pH值对猪卵母细胞体外成熟培养的影响
引用本文:索永善,李跃民,华云芳,肖雄,刘贞伟,靳双星. pH值对猪卵母细胞体外成熟培养的影响[J]. 畜牧兽医杂志, 2005, 24(2): 4-5
作者姓名:索永善  李跃民  华云芳  肖雄  刘贞伟  靳双星
作者单位:西南农业大学,重庆,400716
摘    要:通过用不同pH值的培养观察分析对猪卵母细胞体外成熟培养的影响。结果表明:猪卵母细胞在培养液pH值低于6.5时几乎全部死亡,而pH值达到8,9时死亡率仅为31.7%,经分析,猪卵母细胞对碱性条件的耐受能力比对酸性条件的耐受能力要强。

关 键 词:卵母细胞体外成熟 过用 影响 观察分析 死亡率 培养液 比对 pH值 耐受能力 酸性条件
文章编号:1004-6704(2005)02-0004-02
修稿时间:2004-10-22

Effect of pH on the maturation of swine oocytes in vitro
SUO Yong-shan,LI Yue-min,HUA Yun-fang,XIAO Xiong,LIU Zhen-wei,JIN Shuang-xing. Effect of pH on the maturation of swine oocytes in vitro[J]. Journal of Animal Science and Veterinary Medicine, 2005, 24(2): 4-5
Authors:SUO Yong-shan  LI Yue-min  HUA Yun-fang  XIAO Xiong  LIU Zhen-wei  JIN Shuang-xing
Abstract:The effect of pH on the maturation of swine oocytes in vitro was observed and analyzed by culturing swine oocytes in various medium at different pH. increased to 8. 9,the mortality of oocytes decreased to 31. 7% ;furthermore,when the pH resumed normal,there are still some oocytes developed to mature. These results indicate that the regulation of H+ in media on the permeabglit of swine oocyte's membrane is involved to the phenomenon that the resistance of swine oocyte to alkaline conditions is much better than to acidic conditions.
Keywords:pH  Swine ioocytes
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