AT-hook基因AHL27过量表达延迟拟南芥开花

拟南芥中有29个被称为AHL的蛋白质 (AT-hook motif nuclear localized protein)，但是大多数AT-hook蛋白的功能未知,其中AHL27蛋白含有一个AT-hook基序和一个PPC结构域。AHL27在不同器官的mRNA表达和GUS 组织化学染色分析表明，AHL27主要在根和花中表达；GFP-AHL27亚细胞定位显示AHL27蛋白是一个核定位蛋白。AHL27基因的过量表达，可抑制开花基因FT的表达，同时促进FLC的表达，从而延迟拟南芥在长日和短日条件下的开花时间。研究表明，AHL27基因在拟南芥的生长发育中起重要作用。

Over-expression of AT-hook Gene AHL27 Can Delay the Flowering of Arabidopsis

There are 29 AHL proteins (AT-hook motif nuclear localized proteins) in Arabidopsis, but the functions of their mojorities remain unknown. The AHL27 protein, reported in this study, contains an AT-hook motif and a PPC domain. The analysis of mRNA expression in different tissue organs and GUS histochemical staining showed that AHL27 was mainly expressed in roots and flowers. The sub-cellular localization of GFP-AHL27 indicated that AHL27 was a nuclear protein. AHL27 over-expression could inhibit the expression of flowering gene FT and promote the FLC expression, therefore, delay the Arabidopsis flowering time both under long daylight and short daylight conditions. Our data suggested that AHL27 could play a very important role in Arabidopsis growth and development.