菜用羽衣甘蓝的小孢子胚诱导和植株再生

 以引自日本村田种苗公司的菜用羽衣甘蓝杂交种‘绿叶多汁’为试材, 对小孢子胚诱导条件、胚状体转接时间、生芽培养基和生根培养基进行了优化筛选。在NLN-13 + 0.1 mg·L^{- 1} 6-BA + 0.1mg·L^-1 NAA和NLN-13 + 0.2 mg·L^-1 6-BA + 0.2 mg·L^-1 NAA两种培养基上获得了胚状体, 其发生率分别为1.38胚·蕾^-1和0.98胚·蕾^-1; 转胚时间以接种培养后25 d为宜; 适宜的胚状体生芽培养基为MS +2.0 mg·L^-1 6-BA + 0.1 mg·L^-1NAA + 3%蔗糖+ 0.7%琼脂, 丛生芽诱导率为56138%; 生根培养基以1/2MS + 0.1 mg·L^-1 NAA + 3%蔗糖+ 0.7%琼脂为宜, 生根率100%。

Microspore-derived Embryos and Plant Regeneration in Edible Kale (Brassi-ca oleracea L. var. acephala DC.)

Plant regeneration was firstly reported bymeans of isolated microspore culture in edible kale. A hybrid cultivar named‘Succulent Green Leaf’which was introduced from Japan was used for the experiment. Several combinatory were screened in microspore derived embryos, trans-embryos time, hormone constitution on shooting and rooting in order to obtain the bestway on the regenerative system. Embryoswere attained in the mediums of NLN-13 + 0.1 mg·L ^-1 6-BA + 0.1 mg·L^-1 NAA ( embryo yield per bud was 1.38) and NLN-13 + 0.2 mg·L^-1 62BA + 0.2 mg·L^-1 NAA ( the yield was 0.98). The suitable time for transfer of the embryos was 25 days after inoculation; The best shooting medium was MS+2.0 mg·L^-1 6-BA+ 0.1 mg·L^-1 NAA + 3% sugar + 0.7% agarose. With the shoot induction rate was 56.38% , the suitable medium for rooting was 1/2MS + 0.1 mg·L^-1NAA + 3% sugar + 0.7% agarose and the root inducing rate come up to 100%.